Biocompatible Surgical Meshes From Decellularized Amniotic Membrane And Polyurethane | 41662
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
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Biocompatible surgical meshes from decellularized amniotic membrane and polyurethane

Annual Conference and Expo on Biomaterials

Yabin Zhu, Feng Guan, Jiachang Jing, Qianqian Wei and Lei Hong

Ningbo University, China

Posters & Accepted Abstracts: J Biotechnol Biomater

DOI: 10.4172/2155-952X.C1.050

Meshes play important roles to repair human tissue defect. In this work, human amniotic membrane (HAM) was decellularized and explored the efficacy as an implantable biological mesh. Surfactant, hypertonic saline, lipase and DNAase were used individually or collectively to remove all cell components and remain the extracellular matrix. Results of H&E and DAPI staining demonstrated that the method of surfactant and lipase combining with DNAase is the most effective treatment for HAM decellularization. Primary smooth muscle cells were seeded to evaluate the decellularized HAM’s (dHAM) in vitro cytocompatibility. The in vivo test was performed via implantation at rabbits’ uterus with clinic polypropylene mesh (PP) as the control. The results indicated that dHAM possessed good biocompatibility and will be a potential candidate for biological mesh.

Yabin Zhu has completed her BS degree from Department of Chemistry, Ningbo University, MS degree from Department of Chemistry, Jilin University and PhD from the Department of Polymer Science & Engineering, Zhejiang University, China. She was a Research Fellow in Biomedical Engineering Research Centre (BMERC), Nanyang Technological University, Singapore from 2003 to 2006. She is currently a Professor in the Medical School of Ningbo University from 2006 till date.

Email: [email protected]

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