Journal of Biotechnology & Biomaterials
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Malachite green (100 mg/L) was completely decolorized under static anoxic condition within 4 h by bacteria Bacillus
spp. ETL-2412; however decolorization was not observed at shaking condition. Bacillus spp. ETL-2412 have also shown
decolorization of azo, triphenylmethane and industrial dyes (cotton blue, methyl orange, reactive blue , direct blue, reactive
yellow 81). Semi-synthetic media containing molasses, urea and sucrose have shown 100, 94, 86% decolorization respectively.
Induction in the activities of malachite green reductase and DCIP reductase was observed during MG decolorization suggesting
their involvement in the decolorization process. UV-Visible absorption spectrum, HPLC and FTIR analysis showed degradation
of MG. Toxicity study revealed the degradation of MG into non-toxic products by Bacillus spp. ETL-2412.
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