Journal of Biotechnology & Biomaterials
Open Access

Abstract

As nutritious substrata of the majority of microbiological mediums protein hydrolyzates of various origins serve. On the main components defining nutritional value of protein hydrolyzates for microorganisms, sub-standard eggs is the valuable protein raw materials in production of nutrient mediums meeting requirements of availability and commonality. The fermentative hydrolyzate of sub-standard eggs (FHSSE) developed by us represents uniform fine, hygroscopic powder of light yellow color with the following indicators: the content of the general nitrogen - 0,57%, the content of amine nitrogen - 0,92%, the content of polypeptides - 2,1%. Research of amino-acid structure of the hydrolyzates received by us shows the high contents in FHSSE (mg/100g): of aspartic (423,1) and glutamic (610,4) acids, arginine (270,9), alanine (244,4), phenylalanine (224,5), valine (265,7), leucine (372,2) and serine (319,5). FHSSE-broth and FHSSE-agar on the basis of the hydrolyzate – universal nutrient mediums for receiving accumulative cultures of microorganisms of different species (Brucella abortus, Listeria monocitogenes, Escherichia coli antakon, Bacillus anthracis, Bacillus subtilis, Lactobacillus acidophilus, Pasteurella multjcidabovis, Salmonella cholera suis, Salmonella dublin, Proteus vulgaris, Staphylococcus aureus). They possess rather high sensitivity and provide growth of test strains of microorganisms at crops of 0,1 ml of a microbic suspension from dilution 10-7. Already in 24 h on these mediums preliminary differentiation is possible. Nature of growth of the tested cultures in mediums on the basis of FHSSE was typical for these species of microorganisms, a colony on the dense medium in S-form.

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