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Bioremediation of crude oil was achieved by glycolipid biosurfactant produced by marine bacterium Staphylococcus spp
isolated from the petroleum hydrocarbon contaminated site cuddalore district, Tamil Nadu, India, through the standard
screening and extracting procedure. The optimized physico-chemical conditions for the maximum production of biosurfactant
was 30h incubation, pH 7.0, 35
C temperature, 20ppt salinity, 3% glucose and 1.5% yeast extract as carbon and nitrogen sources
respectively and ionic supplements of 0.3% K
, 0.1% FeSO
, 0.3% CaCO
, 0.2% MgSO
, 0.2% ZnSO
. Extracted partially
purified biosurfactant showed broader stability over a wide range of pH 2-10, temperature up to 70?C, salinity up to 50ppt shows
the ability to withstand almost of the environmental condition and nil antimicrobial activity against bacterial and fungal strains
pre-isolated from coral reef sediment and water samples shows the indigenous microorganism may not harmed therefore the
fertility of the environment. Biosurfactant mediated bioremediation was carried out in conical flask using petroleum hydrocarbon
pollutant and reef sediment consortium, biosurfactants solubilize the pollutants was used as sole source of carbon and energy
by consortia confirmed by turbidometric, CFU and protein content in the media and was compared with the controls. From our
study, isolated biosurfactant has unique property on its emulsification activity, broader stability and must provide promising
environmental friendly treatment technologies for the remediation of hydrocarbons in future.
Senthil Balan S is a Research Scholar doing PhD in Marine Microbiology, Annamalai University, Tamil Nadu, India. Also holding a category as SRF
in ongoing DBT project previously worked in DRDO project for a period of 2 years and totally has a research experience of 5 years. He finished his
UG, PG, M.Phil and doing PhD in Microbiology in well and reputed institutes. Published 3 paper and now 3 papers in review with good impact factors.
His keen interest is on bioluminescence and biosurfactant.
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