Bone Marrow Cells Contribute To Tubular Epithelium Regeneration Following Acute Kidney Injury Induced By Mercuric Chloride | 4770
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Bone marrow cells contribute to tubular epithelium regeneration following acute kidney injury induced by mercuric chloride

3rd World Congress on Biotechnology

Neelam Yadav1, Someshwara Rao, Dipankar M Bhowmik2 and Asok Mukhopadhyay

Posters: J Biotechnol Biomater

DOI: 10.4172/2155-952X.S1.020

Acute tubular necrosis (ATN) caused by renal ischemia, renal hypo-perfusion, or nephrotoxic substances is the most common form of acute kidney injury (AKI). There are few treatment options for this life-threatening disease and the mortality rate exceeds 50%. In critical cases of AKI the only option left is renal transplantation. We report that bone marrow cells (BMCs) are involved in regeneration of kidney tubules following acute tubular necrosis in the mouse. We compared the relative contributions of enhanced green fluorescence protein (eGFP) expressing BMCs in two different approaches to kidney regeneration in the mercuric chloride (HgCl 2 )-induced mouse model of acute kidney injury (AKI): induced engraftment and forced engraftment. The differentiation of donor cells into renal tubular epithelium was examined by flowcytometric and immunohistochemical analyses. In the forced engraftment approach, but not in the induced engraftment approach, BMCs were found to contribute to the regeneration of tubules in the renal cortex and outer medullar regions. About 70% of donor cells expressed megalin. In vitro culture revealed that lineage-depleted (Lin-) BMCs differentiated into megalin, E-cadherin and cytokeratin-19 (CK- 19) expressing renal epithelial cells. These results suggest that in mice Lin- BMCs may be involved to regenerate renal tubular epithelium. Overall, this study demonstrates that transplanted BMCs can contribute to the regeneration of tubular epithelium in the HgCl 2 -induced mouse AKI model.
Neelam Yadav has completed her Ph.D from Industrial Toxicology Research Centre, Lucknow and postdoctoral studies from Stem Cell Biology Laboratory of National Institute of Immunology (NII) New Delhi, India. She is Assistant Professor in Department of Biochemistry, at Dr. R.M.L. Avadh University, Faizabad, India. Dr. Neelam has published many papers in reputed international journals and filed one patent.