Acute tubular necrosis (ATN) caused by renal ischemia, renal hypo-perfusion, or nephrotoxic substances is the most common
form of acute kidney injury (AKI). There are few treatment options for this life-threatening disease and the mortality rate
exceeds 50%. In critical cases of AKI the only option left is renal transplantation. We report that bone marrow cells (BMCs) are
involved in regeneration of kidney tubules following acute tubular necrosis in the mouse. We compared the relative contributions
of enhanced green fluorescence protein (eGFP) expressing BMCs in two different approaches to kidney regeneration in the
mercuric chloride (HgCl
)-induced mouse model of acute kidney injury (AKI): induced engraftment and forced engraftment.
The differentiation of donor cells into renal tubular epithelium was examined by flowcytometric and immunohistochemical
analyses. In the forced engraftment approach, but not in the induced engraftment approach, BMCs were found to contribute
to the regeneration of tubules in the renal cortex and outer medullar regions. About 70% of donor cells expressed megalin.
In vitro culture revealed that lineage-depleted (Lin-) BMCs differentiated into megalin, E-cadherin and cytokeratin-19 (CK-
19) expressing renal epithelial cells. These results suggest that in mice Lin- BMCs may be involved to regenerate renal tubular
epithelium. Overall, this study demonstrates that transplanted BMCs can contribute to the regeneration of tubular epithelium in
-induced mouse AKI model.
Neelam Yadav has completed her Ph.D from Industrial Toxicology Research Centre, Lucknow and postdoctoral studies from Stem Cell Biology
Laboratory of National Institute of Immunology (NII) New Delhi, India. She is Assistant Professor in Department of Biochemistry, at Dr. R.M.L. Avadh
University, Faizabad, India. Dr. Neelam has published many papers in reputed international journals and filed one patent.
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