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Caspase-3 Activity As A Prognostic Factor In Colorectal Carcinoma | 4755
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
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Caspase-3 activity as a prognostic factor in colorectal carcinoma

3rd World Congress on Biotechnology

V. Nagendra Babu, V. S. Tejaswini and P. Anamika

Posters: Agrotechnol

DOI: 10.4172/2155-952X.S1.020

Abstract
Several techniques to determine apoptotic frequencies in tumors have been described. In this study, we report that biochemical detection of enzymatic caspase-3 activity is a simple and quantitative technique to measure apoptosis in colorectal tumor cells. The relevance of the level of apoptosis in colorectal cancer for the clinical course remains unclear. Therefore, we studied the correlation between caspase-3 activity and prognosis of the disease in relation to different factors known to be involved in apoptosis induction. High caspase-3 activity significantly correlated with a higher risk of recurrence and was preferentially found in tumors of the right side of the colon. No correlation was detected between high caspase-3 activity and altered protein expression of p53, beta- catenin, or proteins of mismatched repair genes. This indicates that high caspase-3 activity has no evident correlation with the genetic Wnt-signaling or the mismatch repair mutational pathways. The caspase-3 activity significantly correlated with CD57(+) tumor infiltrating cells. Therefore, high caspase-3 activity in right-sided tumors might be induced by a specific lymphocytic reaction.
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