Journal of Biotechnology & Biomaterials
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Several techniques to determine apoptotic frequencies in tumors have been described. In this study, we report that biochemical
detection of enzymatic caspase-3 activity is a simple and quantitative technique to measure apoptosis in colorectal tumor
cells. The relevance of the level of apoptosis in colorectal cancer for the clinical course remains unclear. Therefore, we studied
the correlation between caspase-3 activity and prognosis of the disease in relation to different factors known to be involved
in apoptosis induction. High caspase-3 activity significantly correlated with a higher risk of recurrence and was preferentially
found in tumors of the right side of the colon. No correlation was detected between high caspase-3 activity and altered protein
expression of p53, beta- catenin, or proteins of mismatched repair genes. This indicates that high caspase-3 activity has no evident
correlation with the genetic Wnt-signaling or the mismatch repair mutational pathways. The caspase-3 activity significantly
correlated with CD57(+) tumor infiltrating cells. Therefore, high caspase-3 activity in right-sided tumors might be induced by a
specific lymphocytic reaction.
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