Dersleri yüzünden oldukça stresli bir ruh haline sikiş hikayeleri bürünüp özel matematik dersinden önce rahatlayabilmek için amatör pornolar kendisini yatak odasına kapatan genç adam telefonundan porno resimleri açtığı porno filmini keyifle seyir ederek yatağını mobil porno okşar ruh dinlendirici olduğunu iddia ettikleri özel sex resim bir masaj salonunda çalışan genç masör hem sağlık hem de huzur sikiş için gelip masaj yaptıracak olan kadını gördüğünde porn nutku tutulur tüm gün boyu seksi lezbiyenleri sikiş dikizleyerek onları en savunmasız anlarında fotoğraflayan azılı erkek lavaboya geçerek fotoğraflara bakıp koca yarağını keyifle okşamaya başlar

GET THE APP

Catabolic Route For 3-guanidinopropionic Acid Utilization By Aspergillus Niger: Involvement Of 4-guanidinobutyrase | 63590
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
Open Access

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
Google Scholar citation report
Citations : 2154

Journal of Biotechnology & Biomaterials received 2154 citations as per Google Scholar report

Indexed In
  • Index Copernicus
  • Google Scholar
  • Sherpa Romeo
  • Open J Gate
  • Genamics JournalSeek
  • Academic Keys
  • ResearchBible
  • China National Knowledge Infrastructure (CNKI)
  • Access to Global Online Research in Agriculture (AGORA)
  • Electronic Journals Library
  • RefSeek
  • Hamdard University
  • EBSCO A-Z
  • OCLC- WorldCat
  • SWB online catalog
  • Virtual Library of Biology (vifabio)
  • Publons
  • Geneva Foundation for Medical Education and Research
  • Euro Pub
  • ICMJE
Recommended Journals
Share This Page

Catabolic route for 3-guanidinopropionic acid utilization by Aspergillus niger: Involvement of 4-guanidinobutyrase

Joint Event on 15th World Congress on Biotechnology And Biotech Industries Meet and 2nd International Conference on Enzymology and Molecular Biology

Tejaswani Saragadam, Sunil Kumar and Narayan S Punekar

IIT Bombay, India

Posters & Accepted Abstracts: J Biotechnol Biomater

DOI: 10.4172/2155-952X.C1.071

Abstract
Aspergillus niger is a metabolically versatile filamentous fungus that utilizes various guanidinium compounds as nitrogen source. The fungus utilizes 4-guanidinobutyric acid (GB), whereas its lower structural homologue 3-guanidinopropionic acid (GP) is very poorly metabolized. The enzyme 4-guanidinobutyrase (GBase) facilitates GB catabolism in this fungus. There is no specific 3-guanidinopropionase (GPase) in A. niger but the purified GBase itself exhibits low GPase activity. Based on these observations we hypothesized that the inability of the fungus to mobilize GP as a nitrogen source is because GP is a poor GBase substrate. Two strategies were employed to test this; one was to increase the mycelial GBase levels and tailoring the GBase specificity towards GP was the second approach. A constitutive expression of GBase in A. niger resulted in normal growth on GP indicating that intracellular GBase levels essentially limit GP utilization in this fungus. There was a direct correlation between growth on GP and cellular GBase levels. In the second approach, altering GBase substrate specificity was attempted. A. niger spores were exposed to ethyl methane sulfonate (EMS) and the mutants were selected through differential growth on GP versus GB. One mutant that better utilized GP than the parent strain was selected and analyzed. Neither an increased GBase activity nor a specific GPase activity was observed in this mutant. Furthermore, no mycelial GPase activity was detected when the mutant was grown on GP. The presence of urea in the spent media when the mutant was grown on GP however implicates a GPase. The possibility of an alternate route for GP catabolism, not involving a GBase needs further study.
Biography

Tejaswani Saragadam is an Integrated MSc-PhD student working under Professor N S Punekar at IIT Bombay. She is working on the aspects of enzymology and metabolism in Aspergillus niger, an industrially well-known fungus for citric acid production and various enzymes. Understanding the nitrogen metabolism in this fungus and studying new pathways and enzymes involved in nitrogen metabolism forms her major work. Further characterizing these enzymes and understanding their role in the novel metabolic pathways forms the basis of her study.

Email: tejaswini.iitb@gmail.com

Top