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CGPSA- Comparative Genomics Methodology For Phylogenetic And Synteny Analysis Of Gene Families In Different Species | 4642
Journal of Biotechnology & Biomaterials
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With the advancement in the scientific genomic research, there is an explosion in the number of completely sequenced
genomes over the past decade, which brought us to the urgency of a well established computational strategy or methodology
to analyse different genomes or different gene families in these genomes. We developed a computational strategy, to compare
different gene families among various plant species. The analysis pipeline comprised of 5 main steps including identification of
genes for the specific gene family, In-silico expression analysis and physical mapping of the identified genes. The phylogenetic
analysis can be performed for gene families among different species and the synteny analysis can be done to identify the
orthologous and non orthologous genes of a gene family among different species. A care was taken on how to represent the huge
sequence data to draw meaningful conclusions. We used this strategy to perform the comparative analaysis of the seven defense
response gene families such as Beta-glucanase and Chitinase in 23 plant species that not only represents two major classes- diocts
and monocots but also belong to very diverse groups like legumes, fruits, vegetables, spices, flowers, medicinal and aromatic
plants , grains, and trees. With this strategy a total of 6864 defense response genes were identified and analysed comparatively in
the 23 plant species. The developed strategy for comparative genomics of any gene or gene family with computational methods
can be used effectively across the genomes.
Hukam Chand Rawal has completed his M.Sc. in Bioinformatics from Jamia Millia Islamia, New Delhi, India and now working as Senior Research
Fellow (on Bioinformatics and Comparative Genomics of crops) at National Research Centre on Plant Biotechnology under Dr. T. R. Sharma.
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