Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
Chemical exchange saturation transfer (CEST) based magnetic resonance (MR) methods are widely used to
probe chemical exchange phenomenon in both model systems as well as in biological tissues
methods probe exchanging spin dynamics and provide endogenous molecular contrast, which among other MR
parameters depends on the concentration and pH of the solute spins.
CEST methods require slow to intermediate exchange on the NMR time scale (chemical shift (
exchange rate (
)), and they primarily probe longitudinal magnetization exchange. The major advantage of the
CEST method is that, depending upon the rates of exchangeable spins, it inherently has an order of magnitude
or greater sensitivity advantage over conventional magnetic resonance spectroscopy (MRS) methods. CEST has
been exploited to assess pH in biological tissues in vivo, to quantifyglycogen in liver, glycosaminoglycans in
cartilage, myoinositol (MI) and glutamate (Glu) in brain, creatine (Cr) in brain, skeletal muscle and myocardium
as well as in studying gene expression, and differentiation between gliomas and radiation necrosis. Recently,
some preliminary results demonstrating the feasibility of measuring pH based cell viability and glucose as a
biodegradable MRI contrast agent have been described
Given the broad spectrum of exchange rates of amide, amine and hydroxyl protons on biologically
important molecules, higher static magnetic field strengths allow a wider range of exchange rates to be probed.
Consequently, the advent of whole body 7T MRI scanners has reinvigorated research activity in CEST mediated
molecular imaging and expanded the range of the metabolites able to be probed
. In addition, there is a
separate class of exogenous agents, known as PARACEST agents that combine exchangeable protons capable
of providing CEST with a paramagnetic metal ion. Since PARACEST agents create larger chemical shifts
between exchangeable protons, CEST experiments involving these agents have significantly reduced direct water
In this presentation, brief outline of the basic principles, technical requirements, sensitivity advantages
and recent developments of CEST methods, and their implementation in measuring endogenous metabolites
in biological systems will be provided. Some representative examples of detecting endogenous metabolites in
pathological conditions such as tumors and Alzheimer?s disease will be described. In addition, advantages and
potential challenges of CEST MRI in quantifying the metabolites under
environments will be discussed.
Ravinder Reddy joined the Scientific Advisory Board in 2005 and is currently a Professor of radiology at the University of
Pennsylvania and a director of the Center for Magnetic Resonance and Optical Imaging, a NIH-funded research center. His
research interests include novel, multinuclear magnetic resonance imaging and spectroscopic techniques for early diagnosis of
cancer and Alzheimer?s disease. He is an active member in the International Society of Magnetic Resonance Imaging in Medicine
and the Osteoarthritis Research Society International, and serves as a referee for the
Journal of Magnetic Resonance and
Magnetic Resonance in Medicine
. Ravinder holds a B.Sc. in math, physics and chemistry from Osmania University in Hyderabad,
India, a M.Sc. in chemistry from Kakatiya University in Warangal, India and a Ph.D. in chemistry from the Indian Institute of
Technology in Kanpur, India.
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals