Chemical Synthesis Of A Recombinant Human Granulocyte Colony Stimulating Factor (rhG-CSF) CDNA And Its Expression Analysis | 17239
Journal of Biotechnology & Biomaterials
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Recently, granulocyte colony-stimulating factor (G-CSF) has been recognized as an important molecule for the treatment
of a wide range of complex ailments such as cancer, AIDS, H1N1 influenza, cardiac and neurological diseases. Such a
vast therapeutic potential of G-CSF has lured the scientists to utilize biotechnological approaches for the synthesis of this
pharmacologically active agent. This study describes the use of a synthetic G-CSF cDNA molecule and its efficient utilization
of producing the target protein by a simple cloning protocol. It was constructed the entire synthetic cDNA using a DNA
synthesizer with the aim to increase its expression level by specific sequence modifications at the 5? end of the G-CSF coding
region and decreasing the GC content without altering the predicted amino acids sequence. The identity of the resulting
protein was confirmed by the highly specific enzyme-linked immunosorbent assay. In conclusion, a synthetic G-CSF cDNA
in combination with the recombinant DNA protocol offers a rapid and reliable strategy for synthesizing the target protein.
However, the commercial utilization of this methodology requires rigorous validation and quality control.
Salman A H Alrokayan has completed his PhD from Nottingham University, United Kingdom. He is the director of bionanotechnologyProgram, Visiting professor
(Leeds University UK). He has 5 patents and published more than 50 papersin reputed journals and has been serving as an editorial board of ?Journal of
Biomaterials and Tissue engineering? as a Regional Editor ? USA.
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