Journal of Biotechnology & Biomaterials
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Tinospora cordifolia plant is known for its many medicinal properties and stem of Tinospora cordifolia plant is mainly used
in ayurvedic preparations. The different class of biologically active compounds such as alkaloids, diterpenoids lactones,
glycosides, steroids, phenolics, sesquiterpenoids, polysaccharides and other aliphatic compounds are reported in the Tinospora
cordifolia plant. The direct analysis in real time (DART) mass spectrometric technique is very much effective in chemical profiling
of natural products as solid or liquid, leaf or stem or any other samples can be directly investigated without prior sample treatment.
In present work we have tried to figure out the presence of important medicinal compounds in the male and female Tinospora
cordifolia plant parts stem and leaf by profiling through the new direct analysis in real time mass spectrometric technique
and authentication of compounds by Q-TOF LCMS and MSMS techniques. Leaf and stem of male as well as female Tinospora
cordifolia plant is subjected under investigation is described in present paper. The results show that the plant type male and
female could be easily differentiated using DART MS data and chemical profiling clearly indicate the presence of characteristic
compound in each part of Tinospora cordifolia plant under investigation. Some medicinally important compounds namely
Jatrorrhizine, Magnoflorine, Tembetarine, Tetrahydropalmatine, Columbin, and β- Sitosterol are found in both male and female
plant by their molecular formula and exact mass measurement. PCA with multivariate analysis clearly discriminate between
male and female of Tinospora cordifolia. PC1 vs PC2 plot which explains 63.87% information with the help of nine marker peaks
shows cent percent discrimination between male and female plants of Tinospora cordifolia. Comparative Chemotaxonomy of all
the plant parts for male and female using QTOF LCMS, MS/MS (ESI and APCI) and DARTMS with their biological activity will
be discussed during the seminar. This is important for quality control and efficacy of herbal drugs.
Brijesh Kumar has completed his PhD from Dr RML Avadh University Faizabad UP, India. He is the Principal Scientist in Sophisticated Analytical
Instrument Facility (SAIF) division of CSIR-Central Drug Research Institute, Lucknow, India a premier drug research organization. He has published
more than 40 papers in reputed journals. His current area of research is application of Mass Spectrometry tools (DARTMS/Q-TOF LCMS/4000
Q Trap LCMS) for qualitative and quantitative evaluation of known and unknown small molecules for quality control and authentication of Indian
Medicinal Plant/parts. He is also involved in identification of marker compounds using statistical software.
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