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Chitosan-lignosulfonatesono-chemically Prepared Nanoparticles: Characterisation And Potential Applications | 17264
Journal of Biotechnology & Biomaterials
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Biodegradable chitosan microspheres have been widely researched as a potential delivery system. In this work, nanoparticles
comprising chitosane-lignosulfonate polyelectrolyte complex were newly developed for potential cosmetic or medical
application, due to their recognized properties of biocompatibility, biodegradability and antioxidative properties. Various
conditions for microspheres preparation were studied and thoroughly characterized by measuring particle size, zeta potential,
polydispersity index. pH of chitosan solution and sonication time were determinant factors on the development of smaller
particles with low polydispersity index(8minutes of sonication at pH 5) in the presence of proper surfactant, poloxamer 407
230 nm). A full characterization was performed in order to assess the beneficial effects of lignosulfonate onto
chitosan microspheres.Lignosulfonates induced higher stability against lysosyme, an enzyme present in human body fluids,
showing good resistance against enzymatic degradation. The morphology was evaluated, as well, by transmission electron
microscopy. These particles were also shown to be able to incorporate a hydrophilic protein model, releasing of RNase Aagainst
0.5 g/L of lysosyme and presenting high rate of release in low concentration of lysosymemedium.
Suyeon Kim obtained her PhD degree in chemical textile department in Minho University in Portugal in 2009. She graduated in textile engineering in Youngnam
University in Korea. Now she is working as researcher at Interdisciplinary Research Laboratory in PUCP and her main research area is the medical biotechnology
and functional polymer synthesis from natural components. She published in around 15 international journals, book chapters and in area of biotechnology, polymer
engineering and enzyme biotechnology. She also has patent in enzymatic processing in textile industry.
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