Comparative Analysis Of Complete Chloroplast Genome Sequence Of Two Aconitum Species And In The Family Ranunculaceae | 60072
Journal of Biotechnology & Biomaterials
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Aconitum species are well known herbaceous medicinal ingredient as well as toxic material and has great economic value in Asian
countries. However, genomic information is still limited in Ranunculaceae. In this study, we completed chloroplast genome
sequence of two Aconitum species, A. coreanum and A. carmichaelii, based on the Illumina MiSeq platform. The gene order, gene
content and orientation of two Aconitum chloroplast genomes exhibit the general structure of flowering plants and are similar to
other Aconitum species. The two Aconitum chloroplast genomes are 155,880 and 157,040 bp in length, respectively and contain 131
unique functional genes including 86 protein coding gene, 8 rRNA and 37 tRNA. We established genetic relationship of Aconitum
species and Ranunculaceae through phylogenetic tree based on 71 protein coding genes of 19 angiosperms. Comparison of the
chloroplast genome structure and gene order to those of Aconitum species revealed general contraction and expansion of the inverted
repeat region (IR) and single copy boundary regions. We obtained barcoding target sequence and developed SCAR marker helpful
for discrimination of the Aconitum species. These results suggest that the sequence variables of chloroplast genome could provide the
useful genetic information and development of molecular marker for discrimination to identify Aconitum species.
Inkyu Park is a Senior Research Scientist at the Korea Institute of Oriental Medicine (KIOM), South Korea. He has completed his PhD from Chungnam National University, Republic of Korea. His research has centered upon chloroplast genome study with development molecular marker and plastid evolution.