Comparative Genomics And Genome Wide Analysis Of Bidirectional Promoters In Candida Albicans | 4888
Journal of Biotechnology & Biomaterials
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Introduction: With the increasing number of genome sequencing data, it is critically needed to understand the coordination
among various functional classes of genes. A genome wide analysis of gene organization and their promoter functions can give
a significant insight into the working of functionally related genes as gene networks. So, with the object of identifying such
organization, we have investigated the genome of Candida albicans for bidirectional promoters and compared it with the data of
bidirectional promoters of human genome.
Summary of Results: Cluster of functionally related genes, which is also called ?Operon?, is a general feature of bacteria and other
prokaryotes but such gene organizations are much less investigated in eukaryotes. The completion of Candida albicans genome
sequencing project provided an unique opportunity to study the distribution of genes throughout the genome. By analysing
the intergenic distances between 6,094 open reading frames, we have identified a class of divergently transcribed gene pairs,
representing more than 20% of the genes in the genome, whose translational start sites are separated by less than 1000 base pairs.
Upon comparative analysis it was found that, it was significant proportion than bidirectional promoters present in human genome.
Although, this bidirectional arrangement has been previously described in various species, the prevalence of bidirectional gene
pairs in C. albicans is striking. Our work also shows that these gene pairs have a variety of functional categories with more than
fifty percent of them having a role in cellular metabolisms such DNA repair. Microarray database analysis indicated that many
bidirectional pairs are co-expressed where they were tend to have positive Pearson correlation coefficient scores. Further, as a
validation of computationally predicted bidirectional promoters, we constructed a dual reporter assay by which we show that
promoter sequence between Nag1 and Dac1 initiate transcription in both directions.
Conclusion: These findings demonstrate that a bidirectional arrangement provides a unique mechanism of regulation and
metabolic adaptation for a significant number of Candida albicans species.
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