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Comparison Of Milk Ring Test With Milk-ELISA For Diagnosis Of Brucellosis | 4742
Journal of Biotechnology & Biomaterials
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Brucella contaminated milk is a potential threat to the new born calves and human beings. Udder and supramammary lymph
nodes are the primary sites of Brucella multiplication in lactating bovine animals and hence become potential source of
contaminants in milk. In response of bacterial multiplication, antibodies are produced by animal and get excreted in the milk.
MRT and Milk-ELISA are reliable tests to detect these secreted antibodies. Organized dairy farm of Pantnagar was the target
populations for present study. A total 246 milk samples of cattle were screened for Brucella antibodies by MRT and Milk-ELISA.
Results showed that, 23.98% and 28.05% cattle population were positive for Brucella antibodies, respectively by Milk-ELISA and
MRT. Detection of antibody in cattle respectively by MRT and ELISA in milk showed that, maximum percentage of crossbred
animals (28.48% and 33.54%) had antibody against Brucella in comparison to indigenous group of animals (15.91% and 18.18%).
Among age groups, 6 to <9 years age groups (33.71% and 38.20%) had maximum reactivity against Brucella followed by ≥9 years
age groups (20.24% and 22.62%) and 3 to <6 years (16.44% and 21.92), respectively by MRT and Milk-ELISA. Results of this
study revealed, brucellosis were significantly prevalent in target population. Statutory mass vaccination programme in susceptible
animals are only method of control and eradication of disease since slaughter of cattle are banned in our country.
Anand Mohan completed his B.V.Sc & A.H. form GADVASU, Ludhiana (Punjab) and M.V.Sc. from GBPUA&T, Pantnagar (Uttarkhand) in Department
of Veterinary Epidemiology and Preventive Medicine under ICAR-JRF with distinction. Now he is pursuing Ph.D in Veterinary Bacteriology from IVRI,
Izatnagar (U.P.) under UGC-JRF. His two papers have been accepted for publication in Indian journals. He published fifteen abstracts in different
National/International Conferences/Symposium. He had been awarded NCC-B certificate in 2006.
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