Compensatory Effects Of HOGG1 For HMTH1 In Oxidative DNA Damage Caused By Hydrogen Peroxide | 53229
Journal of Biotechnology & Biomaterials
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This study aimed to investigate the potential compensatory effects of hOGG1 and hMTH1 in the repair of oxidative DNA
damage. The hOGG1 and hMTH1 gene knockdown human embryonic pulmonary fibroblast cell lines were established
by Lentivirus-mediated RNA interference. The messenger RNA (mRNA) levels of hOGG1 and hM1TH1 were analyzed by
the real-time polymerase chain reaction and 8-hydroxy-20-deoxyguanosine (8-oxo-dG) formation was analyzed in a highperformance
liquid chromatography-electrochemical detection system. The hOGG1 and hMTH1 knockdown cells were
obtained through blasticidin selection. After transfection of hOGG1 and hMTH1 small interfering RNA, the expression levels
of the mRNA of hOGG1 and hMTH1 genes were decreased by 97.2% and 96.2%, respectively. The cells then were exposed to
100 mmol/L of hydrogen peroxide (H2O2) for 12 hours to induce oxidative DNA damage. After H2O2 exposure, hMTH1 mRNA
levels were increased by 25% in hOGG1 gene knockdown cells, whereas hOGG1 mRNA levels were increased by 52% in hMTH1
gene knockdown cells. Following the treatment with H2O2, the 8-oxo-dG levels in the DNA of hOGG1 gene knockdown cells
were 3.1-fold higher than those in untreated HFL cells and 1.67-fold higher than those in H2O2-treated wild-type cells. The
8-oxo-dG levels in hMTH1 gene knockdown cells were 2.3-fold higher than those in untreated human embryonic pulmonary
fibroblast cells but did not differ significantly from those in H2O2-treated wild-type cells. Our data suggested that hOGG1 could
compensate for hMTH1 during oxidative DNA damage caused by H2O2, whereas hMTH1 could not compensate sufficiently
for hOGG1 during the process.
Yuebin Ke is a Professor of the Shenzhen Center for Disease Control and Prevention and an Adjunct Professor of Life Sciences at Shenzhen University. He has completed his PhD from Huazhong University of Science and Technology and Postdoctoral studies from Virginia Polytechnic Institute and State University. He has published 18 papers in the areas of environmental health and molecular biology.