Journal of Biotechnology & Biomaterials
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Qualitative analysis with colistin and streptomycin on the growth curves of the P. syringae Lz4W shows that the growth was
resumed to exponential stage after delayed lag phase at a lethal antibiotic concentration, when supplied externally with
prepared OMVs from the same organism. This result shows that the OMV?s may play an important in antibiotic resistance. In
order to understand the functions of the OMVs, a detailed study was undertaken to analyze different components present in the
OMVs. We have characterized different components of OMVs from an Antarctic bacterium Pseudomonas syringae Lz4W. The
OMVs were prepared from P. syringae Lz4W and their size was determined by Transmission Electron Microscopy (TEM) and
dynamic light scattering. The phospholipids from these MVs were characterized by a combination of MALDI-MS and fatty-acid
methyl ester (FAME) analysis on GC-MS. The OMVs were also found to contain LPS. The proteins of OMVs were separated by
1D-gel and then analyzed by nano LC-MALDI-TOF/TOF and LC-ESI-MS/MS (Orbitrap). Mass spectrometric data was analyzed
by Mascot and Sequest softwares respectively. Since the genome sequence of this organism is not known, a database was prepared
from different Pseudomonas species and used to identify the proteins. The subcellular localizations of these proteins were
predicted by using Psortb V3 software. Along with inner membrane, periplasmic and outer membrane proteins, we identified
many cytoplsmic proteins/enzymes segregating in to the OMVs. These proteins are implicated in their functions. Attempts were
also made to understand the mechanism of protection to bacteria by the OMVs using fluorescent probe NPN.
Medicharla V. Jagannadham completed his PhD in the year 1998 and working as a Scientist for the past 22 years at Centre for Cellular and Molecular
Biology, Hyderabad, India. He has published more than 35 research papers in internationally reputed Journals. He published a monograph on
the ?Membrane proteins of an Antarctic bacterium Pseudomonas syringae? He is a member of several learned scientific societies such as Society
of Biological Chemists (India), The American Society for Biochemistry and Molecular Biology. His research interests are developing proteomics
technologies and studies on the functions of the outer membrane vesicles of bacteria.
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