Dersleri yüzünden oldukça stresli bir ruh haline sikiş hikayeleri bürünüp özel matematik dersinden önce rahatlayabilmek için amatör pornolar kendisini yatak odasına kapatan genç adam telefonundan porno resimleri açtığı porno filmini keyifle seyir ederek yatağını mobil porno okşar ruh dinlendirici olduğunu iddia ettikleri özel sex resim bir masaj salonunda çalışan genç masör hem sağlık hem de huzur sikiş için gelip masaj yaptıracak olan kadını gördüğünde porn nutku tutulur tüm gün boyu seksi lezbiyenleri sikiş dikizleyerek onları en savunmasız anlarında fotoğraflayan azılı erkek lavaboya geçerek fotoğraflara bakıp koca yarağını keyifle okşamaya başlar


Construction Of Broad Host Range Vector For Expression Of Heterologus Protein In Brucella Abortus Strain 19 | 4729
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
Open Access

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
Google Scholar citation report
Citations : 2154

Journal of Biotechnology & Biomaterials received 2154 citations as per Google Scholar report

Indexed In
  • Index Copernicus
  • Google Scholar
  • Sherpa Romeo
  • Open J Gate
  • Genamics JournalSeek
  • Academic Keys
  • ResearchBible
  • China National Knowledge Infrastructure (CNKI)
  • Access to Global Online Research in Agriculture (AGORA)
  • Electronic Journals Library
  • RefSeek
  • Hamdard University
  • OCLC- WorldCat
  • SWB online catalog
  • Virtual Library of Biology (vifabio)
  • Publons
  • Geneva Foundation for Medical Education and Research
  • Euro Pub
Recommended Journals
Share This Page

Construction of broad host range vector for expression of heterologus protein in Brucella abortus strain 19

3rd World Congress on Biotechnology

Justin Davis K

Posters: Agrotechnol

DOI: 10.4172/2155-952X.S1.020

Brucellosis, a major zoonotic disease causing abortion and infertility in cattle, is controlled mainly by vaccination with strain Brucella abortus strain 19. It is a smooth, attenuated and live vaccine candidate which can induce a strong humoral and cellular immune response and therefore, it is an attractive vector platform for the delivery of heterologus antigens. The objective of this study was to construct a broad host range vector for expression of heterologus protein in B abortus strain 19. For this, promoter sequence of Brucella groE gene was cloned into broad host range vector pBBR 122. For one step purification and easy detection of the expressed protein, six histidine tag was added in to the reverse primer of groE promoter. To check promoter activity and expression status of the construct, a promoterless GFP gene was cloned into the vector. The construct pBBR GroE_ GFP was then electroporated in to strain 19. The expression of GFP was induced by heat shock at 42 o C and treatment with H 2 O 2 . Then the recombinant protein was purified by using urea lysis method and followed by Ni-NTA affinity chromatography. Further, the purified protein was dialysed and the specific reactivity of the recombinant protein was checked by western blotting using anti histidine antibody. A single specific band could be detected at 28kDa confirming the expressed recombinant protein. Based on the present study, it is concluded that B abortus strain 19 can be used as vector for expression of foreign protein and further experimentation is required study its vaccinal potential.
Justin Davis K is the native of Kerala, India. He has completed his MVSc degree from Indian Veterinary research institute in veterinary epidemiology and has procured PhD from the same institute on Veterinary Bacteriology. He had received IVRI fellowship for both MVSc and PhD. He had also received PTF grant for young scientists for presenting a paper at Estonia conference.