Coupled Processing Of Saccharomyces Cerevisiaes No RNA 5? And 3? Termini | 17154
Journal of Biotechnology & Biomaterials
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Small nucleolar RNAs (snoRNAs) are the best characterized non-coding RNAs transcribed by RNA polymerase II. They are
produced as precursors, whose extended 3? ends are trimmed exonucleolytically, whereas 5? ends either undergo combined
endo- and exonucleolytic processing by Rnt1 and Rat1, respectively, or remain unchanged. In the latter case, the m7G cap
becomes hypermethylated by Tgs1. cRT-PCR analyses suggest that processing of snoRNA 3? and 5? termini is tightly coupled.
Inhibition of 5? end maturation, in rnt1Δ strain, cause the accumulation of snoRNA precursors that don?t possess mature 3?
ends but carry polyadenylated extensions. This defect is further increased when components of the cap-binding complex
(CBC) or methyltransferase Tgs1 are missing. Although we observe an accumulation of pre-snoRNAs in rnt1Δ strain, there
are also mature snoRNAs present. These results suggest the existence of an alternative pathway of 5? end processing that most
likely involves pre-snoRNA cap removal by Dcp1/Dcp2 complex. Interestingly most pre-snoRNAs that accumulates in dcp2Δ
strain are not cleaved by Rnt1, which was confirmed by 5 ́ RACE analysis. Obtained data strongly suggest the existence of a
quality control mechanism that coordinates 5? and 3? end processing.
Żaneta Matuszek is a student of the College of Inter-faculty Individual Studies in Science and Mathematics at the University of Warsaw (Poland). Since 2011 she
has been working in the Institute of Genetics and Biotechnology, University of Warsaw, in a lab of RNA metabolism in Eukaryotic cells. She is the organizer of the
International Conference Aspects of Neuroscienceheld annually in Warsaw and the editor of the students? magazine of the same title.
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