Cryopreservation For The Cell Selection Using Water Channels AQPs Combination With Ultra-quick Freezing | 17248
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
Recommended Conferences

6th International Conference on Vaccines and Immunology

Geneva, Switzerland

3rd European Congress on Virology

Geneva, Switzerland
Google Scholar citation report
Citations : 1600

Journal of Biotechnology & Biomaterials received 1600 citations as per Google Scholar report

Indexed In
  • Index Copernicus
  • Google Scholar
  • Sherpa Romeo
  • Open J Gate
  • Genamics JournalSeek
  • Academic Keys
  • ResearchBible
  • China National Knowledge Infrastructure (CNKI)
  • Access to Global Online Research in Agriculture (AGORA)
  • Electronic Journals Library
  • RefSeek
  • Hamdard University
  • OCLC- WorldCat
  • SWB online catalog
  • Virtual Library of Biology (vifabio)
  • Publons
  • Geneva Foundation for Medical Education and Research
  • Euro Pub
Share This Page

Cryopreservation for the cell selection using water channels AQPs combination with ultra-quick freezing

5th World Congress on Biotechnology

Yasuhiro Kato

Posters: J Biotechnol Biomater

DOI: 10.4172/2155-952X.S1.028

Cryopreservation has been only empirically related to cell type and freezing conditions such as cooling rates and freezing solutions. In this report, we found that membrane damage caused by ultra-quick freezing is rescued by expression of aquaporin (AQP), leading to freezing tolerance. It is suspected that AQP can contribute to the improvement in cryopreservation by increasing membrane water permeability even under very low temperatures since AQP-mediated facilitated diffusion of water molecules is temperature independent.It was then examined if the freezing tolerance associated with AQP expression could be applied as a cell selection method. The cells expressing AQP either exogenously or endogenously were exclusively selected after ultra-quick freezing and thawing.Finally it is showed that CHO cells transiently transfected with Endothelin receptor A and AQP4 were also selected and concentrated by multiple cycles of freezing/thawing, which was confirmed with calcium imaging in response to endothelin.Taken together, it is proposed that this simple but efficient and safe method may be applicable to the selection of mammalian cells for applications in regenerative medicine as well as cell-based functional assays or drug screening protocols. Interestingly, also was discovered that multiple steps of ultra-quick freezing/thawing exclusively selects only stably transfected cells, which imply that stably transfected cell lines can be mimicked by this strategy.Taken together, this simple but efficient and safe method may be applicable to the selection of mammalian cells for applications in drug screening protocols as well as cell-based functional assays.
Yasuhiro Kato has completed his PhD at the age of 27 years from Nagoya University and postdoctoral studies from JSPS (Japan Society for the Promotion of Science) and Tokyo Dental College. He is the assistant professor at Keio University School of medicine. He has published some papers for function of membrane protein and development of cell freezing methods using Aquaporin water channels.