C-SLGE- Cloning Of Saccharomyces Cerevisiae Lipase Gene In E.coli Nissle 1917(probiotic) | 12163
Journal of Biotechnology & Biomaterials
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Lipase enzymes can increase the nutritional value of food and perform essential roles in the digestion, transport and processing
of dietary lipids (e.g. triglycerides, fats, oils) in most, if not all, living organisms. Escherichia coli Nissle 1917 is spore forming
probiotic bacteria. In present study, we aimed to clone and express lipase gene in E.coli Nissle 1917 to create better probiotics.
The lipase gene from Saccharomyces cerevisiae was cloned and inserted into vector pUC18 and then transferred into E. coli by
transformation. Insert and PCR analysis of pUC18 from recombinant E.coli Nissle 1917 confirmed the Lipase gene fragment on
agarose gel electrophoresis and found to be nearly 1.4 kb. Lipase gene cloned in E.coli Nissle 1917 showed Lipase enzyme activity
when grown on medium supplemented with lipid source. Recombinant E.coli Nissle 1917 tested for lipase activity on test plates
at various pH (4, 5, 6, 7, 8, 9, 10) to obtain any changing in pH optimum of the enzyme in the new host and Lipase activity was
observed more on the plates of acidic PH than basic and optimum PH was found to be 6
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