Cytotoxicity Of Molybdenum Trioxide Nanoplates | 106704
Toxicology: Open Access
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Introduction & Aim: Nanostructured molybdenum trioxide (MoO3-NPs) is promising material in many applications, in
coatings, plastics, textiles, pigments, lubricants, ceramics and glass production, as antimicrobial agents and for the detection of
Dopamine in pharmaceutical and clinical preparations. In recent years, it was noted that in the case of nanomaterials should
carefully evaluate the risks of their use, as they may pose a health risk. The main aim of this study was to assess the cytotoxic
activity of MoO3-NPs in human pulmonary cells.
Methods: The cytotoxicity of MoO3-NPs was assessed on the alveolar carcinoma epithelial cells (A549) and normal bronchial
epithelium cells (BEAS-2B) after short and long-term time of exposure. Cytotoxicity studies included the effect of MoO3-NPs
on cell viability, cell membrane integrity (NRU assay), mitochondrial metabolic activity (MTT assay) and the ability of the cells
to proliferation (Clonogenic assay).
Results: MoO3-NPs induced a dose- and time-related negative effect on the viability of both kids of the cells in the cytotoxic
doses range 50-300 ?g/ml, depending on cytotoxicity endpoint. In long-term exposure (7 day), MoO3-NPs at concentrations
about 100 ?g/ml impaired proliferations, implying their potential chronic toxicity. A549 cells were less sensitive than BEAS-2B
one, to all measurement parameters.
Conclusion: The sensitivity of BEAS-2B cells to MoO3-NPs is of particular concern. These cells form a defense line of the body
against the penetration of particles into lungs. Inhibition of the ability of BEAS-2B cells to proliferate under the influence of
MoO3-NPs may be an unfavorable phenomenon for predicting their long-term effects of exposure.
Lidia Zapor is currently working in the Department of Chemical, Aerosol and Biological Hazards in the Central Institute for Labour Protection, National Research Institute (CIOP-PIB). She is the Head of Laboratory of Toxicology. The main area of her professional interest is problems of human in the working environment and the toxicity of chemical substances as well as methods of estimating the toxicity of substances in vitro.