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In Association with
Degradation of Poly Aromatic Hydrocarbon (PAH) by biosurfactant producing bacterial strains
6th World Congress on Biotechnology
Suresh Deka1, Kaustuvmani Patowary1 and Mohan Chandra Kalita2
1Institute of Advanced Study in Science and Technology, India 2Gauhati University, India
ScientificTracks Abstracts: J Biotechnol Biomater
Abstract
Polycyclic aromatic hydrocarbons (PAHs) are toxic compounds. Due to leakage or spillage of crude oil, these toxic hydrocarbons may contaminate the sites. These compounds are low water soluble; as a result, they are not easily degraded by microorganisms. So, remediation of PAH’s through seeding only hydrocarbon degrading microorganisms take more time. Microbial produced surfactant can enhance the bioavailability of these hydrophobic compounds for bioremediation. Keeping this in mind, an investigation was carried out to develop an efficient consortium of biosurfactant producing bacterial strains that could be used for remediation of PAH from contaminated sites. For that, a total of twenty three bacterial strains were isolated from hydrocarbon contaminated soil. The ability of degradation of hydrocarbon of the isolates was tested individually and five of them were selected for further study on the basis of utilization of hydrocarbon. Among these five isolates, three were found biosurfactant producer and two non-producers. Ten different consortia were designed involving best hydrocarbon utilizing strains taking both biosurfactant producers and non-producers. The consortium consisting bacterial strain Bacillus pumilus KS2 and Bacillus cereus R2 (identified by 16s rDNA sequencing) had shown best results in degradation of crude oil. The consortium could degrade 82.15% of total petroleum hydrocarbon (TPH) from the crude oil in five weeks of incubation time. The consortium could degrade different PAH’s like naphthalene, fluorene, phenanthrene, anthracene, 3.beta.-Myristoyl Olean-12-en-1, 1H-indene, 2,3-dihydro-1,1,5,6 and their derivatives which indicates the prospective of the consortium to be used for remediation of petroleum hydrocarbon contaminated sites.Biography
Suresh Deka is currently working as a Professor in the Environmental Biotechnology Laboratory, Resource Management and Environment Section, Life Sciences Division, Institute of Advanced Study in Science and Technology (IASST), India. He has published more than 40 research papers in reputed journals, 6 chapters of books and 7 proceeding of seminars. He has completed his Postdoctorate Research on Biosurfactant in University of Ulster, UK under the supervision of Professor I M Banat. He has completed several research projects funded by different central governmental agencies as Principal Investigator and Co-Principal Investigator.
Email: sureshdeka@yahoo.com
