Delayed Seroconversion To STLV-1 Infection Is Associated With Mutations In The Pol And Rex Gene | 4779
Journal of Biotechnology & Biomaterials
Like us on:
Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
Two Cercopithecus aethiops tantalus and 2 Erythrocebus patas monkeys from Central African Republic were transfused
with intraspecies whole blood from monkeys naturally infected with simian T-cell lymphoma/leukemia virus -1 (STLV-1).
Followup studies conducted over 2 years indicated that all 4 monkeys remained healthy despite being infected with STLV-1, as
determined by PCR, cloning and sequencing analyses. ELISA and Western blot analyses indicated that both patas monkeys
seroconverted within 2 months of transfusion, while one tantalus monkey required one year to seroconvert and the other never
fully seroconverted. This latter animal failed to react to HTLV-1 p24 gag antigen. Sequence analyses indicated that, while unique,
the deduced p24 gag amino acid sequence of the STLV-1 Tan 90 strain used to infect this animal was still highly homologous to
the HTVL-1 p24 gag amino acids present in the ELISA and WB assays. However, a mutation in the pol sequence of STLV-1 Tan
90 encoded a putative stop codon, while a common deletion in the pol/rex regulatory gene causes significant changes in the Pol,
and Rex p24 proteins. These same mutations were also observed in the viral DNA of both recipient infected tantalus monkeys
and were not present in the STLV-1 Pat 74 strain. The data suggest that seroconversion to STLV-1 infection may be prolonged
due to such mutations, and that compensatory molecular events vis-a-vis the pol stop condon must have occurred to allow for
Syamalima Dube completed her Ph.D in Biochemistry from Calcutta University. She did postdoctoral studies in University of Chicago and Fox Chase
Cancer Center, Philadelphia. She was a research Assistant Professor in the Department of Medicine, University of Washington, Seattle. Currently
she is a Research scientist in the Department of Medicine, Upstate Medical University, and Syracuse, NY. She has published more than 80 papers
in reputed journals like PNAS, JBC, and New England Journal of Medicine.
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals