Delivery Of Mycobacterium Tuberculosis Lipids Using Chitosan Nanoparticles Induce Potent Cytokine And Antibody Response Through Activation Of γδ T-cells In Mice | 51370
Journal of Infectious Diseases & Therapy
Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
Activation of cell mediated and humoral immune responses to Mycobacterium tuberculosis (Mtb), the causative agent of
tuberculosis (TB), are critical for protection. For decades, most studies regarding immunity to TB are focused mainly on
proteins. In recent years, increasing evidences have shown that Mtb cell wall lipids act as potent adjuvants as well as antigens capable
of activating specific T-cells through their presentation by CD1 molecules and also induce IgM, IgA and IgG antibody responses.
However unlike proteins, delivery and presentation of lipid antigens is a major challenge. Herein, we have used chitosan nanoparticles
(NPs) as Mtb lipid delivery system and showed that chitosan NP mediated delivery of Mtb lipids induce potent cytokine and antibody
responses in immunized mice. Chitosan NP delivered Mtb lipids induced the release of most prominent cytokines associated with
Th1 (TNF-α, IFN-γ, IL-2) and Th2-type (IL-4, IL-5, IL-6, IL-13) immune responses in mice lymphatic and spleen cells as compared
to immunogenic Mtb purified protein derivative (PPD) and chitosan NPs alone. Moreover, mice immunized with Mtb lipid coated
chitosan NPs showed significantly higher levels of IgG, IgG1 and IgM and a moderate increase in IgG2a antibodies as compared to
Mtb lipid liposomes and chitosan NP immunized mice. In conclusion, this study represents a promising new strategy for efficient
delivery of Mtb lipids to trigger enhanced cell mediated and antibody response against Mtb infection.