Design Of Synthetic-hybrid Bacteriocins From Enterocin E50-52 And Pediocin PA-1 For Therapeutic Applications | 39003
Journal of Biotechnology & Biomaterials
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Two hybrid bacteriocins EP and PE were designed by combining the N-terminal of enterocin E50-52 and the C-terminal of
pediocin PA-1 and vice-versa, respectively. Both hybrid bacteriocins showed reduced Minimum Inhibitory Concentration
(MIC) which were 64- and 32-folds lower than pediocin PA-1 and 8- and 4-folds lower than enterocin E50-52, respectively.
Effect of these bacteriocins on transmembrane electrical potential (Δψ) and their ability to induce efflux of intracellular ATP
was investigated. Enterocin E50-52, pediocin PA-1 and hybrid bacteriocin PE were able to dissipate Δψ but EP was unable to
deplete this component. Both hybrid bacteriocins showed loss of intracellular concentration of ATP. EP, however, showed faster
efflux as compared to PE and enterocin E50-52. Enterocin E50-52 and hybrids PE, and EP were active against tested Grampositive
and Gram-negative bacteria such as Micrococcus luteus, Salmonella enteritidis 20E1090, and E. coli O157:H7. The hybrid
bacteriocins designed and described herein are antimicrobial peptides with lower MIC, which induce loss of intracellular ATP,
capable of inhibiting Gram-negative bacteria and PE which dissipates electrical potential. In this study, a hybrid bacteriocin
(PE) of pediocin PA-1 and enterocin E50-52 indicated a 64-fold drop in MIC as compared to its natural peptide counterpart.
Inhibition of Gram-negative pathogens confers an additional advantage for the application of these peptides in therapeutics.
Santosh Kumar Tiwari has completed his PhD from University of Delhi South Campus, New Delhi. He was Visiting Scientist in School of Biological Sciences, Rutgers State University, New Jersey, USA. Currently, he is Assistant Professor in Department of Genetics, Maharshi Dayanand University, Rohtak, Haryana. He has published more than 15 papers in reputed journals and edited a book entitled “Current Trends in Biotechnology”. He has 7 years of teaching and research experience. He was awarded Indo-US Research Fellow, DST Young Scientist and several research projects from UGC, CSIR and DBT, New Delhi.