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Detection And Molecular Characterization Of Pasteuria Penetrans Isolates Parasitizing Root-knot Nematodes In Al-Qassim Area, Saudi Arabia | 17242
ISSN: 2155-952X
Journal of Biotechnology & Biomaterials
Open Access
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Pasteuria penetransis
an endospore-forming bacterial parasite of
Meloidogynespp
. This organism is among the most
promising agents for the biological control of root-knot nematodes. Universal primers for the 16S rDNA gene were used
to amplify DNA from four isolates of
P. penetrans
infecting
M. incognita
in Al-Qassim area. The results indicated that the two
Primers (617F/1166R and 27F/1388R) detected the four isolates of
Pasteuria
from
M. incognita
in Al-Qassim area. A nucleotide
sequence was obtained for the gene encoding the 16S rDNA from each isolate. The sequences of 16S rDNA of four isolates
showed that P. penetrans was found. Comparisons between isolates were investigated using ITS-RFLP. Polymorphic ITS-RFLP
patters from the four restriction endonucleases allowed differentiation of the isolates of
P. penetrans
. Based on cluster analysis
of the obtained fragments of ITS-RFLP, the isolates of
P. penetrans
fall into two distinct clusters. Because of the high specificity
and sensitivity of ITS-RFLP, it was possible to discriminate between
P. penetrans
isolates.
Biography
Suloiman Al-Rehiayani is a professor at Plant Production and Protection Department, College of Agriculture and Veterinary Medicine, Qassim University, Saudi
Arabia. His research interest includes: Design, development and evaluation of agricultural machinery, Mathematical analysis for predict the performance of
agricultural machinery and Development of food processing equipment. He published more than 20 research article in repute journals.
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