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Determination Of Heavy Metal Stress On Bacterial Diversity | 4710
Journal of Biotechnology & Biomaterials
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In the present study total thirty heavy metal resistant bacteria were isolated from sewage of industrial effluents, garages and
petrol pumps of Cachar district of Assam, India, against copper, zinc, and lead. Samples were streaked on selective media?s and
incubated at 370C for 24 hrs for recovery of potent isolates. The isolated and distinct colonies were sub-cultured and obtain in
the form of pure culture and identified on the basis of their morphology and biochemical characters. There were decreases
in total count and microbial population diversity with increasing metal concentrations and the predominant isolates obtained
were Pseudomonas sp., Klebsellia sp. and Bacillus sp. Some isolates exhibited high resistance to heavy metals with minimum
inhibitory concentration (MIC) for heavy metals as 50μg/ml (for copper), 180 μg/ml (for lead) and 1500μg/ml (for zinc). They
also showed multiple heavy metal tolerance and were multi antibiotic resistant.
Soumitra Nath is a Research Scholar from Bioinformatics Centre, Gurucharan College Silchar under DBT funded project and registered for his Ph.D
in Department of Microbiology, Assam University, Silchar (AUS). He has completed his Master degree from Department of Biotechnology, AUS and
PG Diploma in Bioinformatics from Department of Life Science, AUS. He has also been appointed as Lecturer in Department of Biotechnology and
Bioinformatics Centre of Gurucharan College, Silchar. His research aim is to find an efficient soil-bacteria for better crop production in heavy metal
contaminated crop field.
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