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Development and characterization of microsatellite markers in Attalea speciosa and their transferability to six other Attalea species
4th International Conference on Advances in Biotechnology and Bioscience
Lorena Ramos da Mata
Embrapa Genetic Resources and Biotechnology, Brazil
Posters & Accepted Abstracts: J Biotechnol Biomater
Abstract
Babassu (Attalea speciosa Mart. ex Spreng) is a native palm tree with wide distribution in Brazil, where it is one of the main extractive products. The nut extracted from this palm is used in the oil manufacture with domestic and industrial utility. The sustainable use of babassu is important for the preservation of this forest resource and also for the economy of endemic regions of the genus. Microsatellite primers were developed for the main species of Attalea. Total genomic DNA library enriched for TC repeats was constructed for A. speciosa following a standard protocol, and 84 markers were developed. We evaluated the transferability of the 84 microsatellite markers for six other babassu species: A. barreirensis, A. eichleri, A. funifera, A. maripa, A. phalerata and A. vitrivir. The transferability was superior to 63% in all species studied. The markers were characterized in populations of seven Attalea species with 20 to 44 individuals each. The total number of alleles over all loci, observed and expected heterozygosity was similar for the seven Attalea species, ranging from 6.2 to 9.4, 0.64 to 0.74, and 0.48 to 0.56, respectively. These values are comparable to the values obtained for other palms and showed that the seven Attalea species has high genetic diversity. Cluster analysis showed groupings of plants according to their species and also showed that some of the plants are hybrids (A. speciosa x A. eichleri). The set of markers developed constitutes a powerful tool for genetic analysis in the genus Attalea.Biography
Lorena Ramos da Mata has completed her Graduation in Pharmaceutical Sciences at the University of Brasilia in 2008 and MSc in Botany at the same university in 2016. She is currently an Analyst at the Brazilian Agricultural Research Company-EMBRAPA CENARGEN.
E-mail: lorena.mata@embrapa.br
