alexa Development And Evaluation Of Novel PCR Assays For The Sensitive, Rapid And Accurate Detection Of Salmonella Spp., L. Monocytogenes, E. Coli O157:H7 And G. Stearothermofillus In Pasteurized Milk And Fresh Juice Products
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading Please wait..

5th World Congress on Biotechnology
June 25-27, 2014 Valencia Conference Centre, Valencia, Spain

A Scorilas, A Kladi-Skandali, D Ghikas, F Gaitis, E Beletsiotis, K Kalantzi and M Avgeris
Posters: J Biotechnol Biomater
DOI: 10.4172/2155-952X.S1.028
Foodborne illnesses are related with high rates of morbidity and mortality as well as with substantial economic burden. The replacement of traditional and laborious protocols by sensitive and rapid methods is required, and PCR-based assays represent the corner stone of this effort. A multiplex PCR assay was developed for the simultaneous detection of Salmonella spp., Listeria monocytogenes and Escherichia coli O157:H7, using specific primers which target rseB, prfA, and eaeA , respectively. Quantitative TaqMan-based Real-time PCR (qPCR) methodology was developed for their quantification as well as for Geobacillus stearothermofillus . Pasteurized milk and fresh orange juice were spiked and cultured based to ISO methods. Samples were taken for DNA extraction and PCR analysis every two hours until 8 hours of culture. Assay specificity was individually assessed, using DNA from closely related species. Concerning singleplex qPCR, the detection limits for Salmonella spp and G. stearothermofillus were 5x10-4 ng DNA, while for E. coli O157:H7 and L. monocytogenes were 2.5x10 -4 ng DNA. Using multiplex PCR assay, detection of the pathogens was succeeded following 4 hours of culture spiked with 10 cfu/ml or more, while accurate quantification of the pathogens was also performed for the same samples and all the spiked pathogens. We developed two specific and sensitive PCR assays for the simultaneous detection of three different food-associated pathogens, paving the way for their application in food analysis. Acknowledgements: The present work was funded by SYNERGASIA 2009 PROGRAMME. This Programme is co-funded by the European Regional Development Fund and National Resources. (Project code: 09SYN-22-977). Partial expenses for the presentation of this abstract were funded by the University of Athens Special Account of Research Grants no 10812
image PDF   |   image HTML

Relevant Topics

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version