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Development Of A Novel Fermentation And Formulation Process For An Endophytic Beauveria Bassiana Strain | 17055
ISSN: 2155-952X
Journal of Biotechnology & Biomaterials
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There is an increasing demand for alternative or complementary crop protection strategies. A novel approach could be
the use of the entomopathogenic and endophytic fungus
Beauveria bassiana
isolate ATP-02. To use the endophyte as a
commercial biocontrol agent, the fungus has to be mass-produced.
B. bassiana
was raised in shake flask cultures to produce
submerged conidiospores (SCS) which are reported to show a higher shelf life than mycelium and blastospores (BS). It was
found that in mineral media with 5% sugar beet molasses
B. bassiana
produced 0.1x1010 SCS/g sucrose in 192 h. By adding
50 g/L NaCl 48 h after inoculation the SCS yield increased to 1.4x10
10
SCS/g sucrose. The scale-up to a 2 L stirred tank reactor
was carried out at 25?C, 200-600 rpm and 1 vvm at pH 5.5. A total spore yield of 5.2x10
10
spores/g sucrose corresponding to
a SCS yield of 0.2x1010 SCS/g sucrose was obtained after 216 h. Also the yield of SCS increased to 1.1x10
10
SCS/g sucrose by
the addition of NaCl. After fermentation B. bassiana was formulated in a novel spray formulation that delivers the fungus on
oilseed rape leaves, increases persistence, germination and growth on leaves as well as penetration, colonization and efficacy
in bioassays with
Plutella xylostella.
Biography
Rieke Lohse (MSc) is working at the Faculty of Engineering Science and Mathematics in the research group of Prof. Dr. Anant Patel at the University of Applied
Sciences Bielefeld, Germany. From 2005 to 2010, she studied Molecular Biotechnology at the Bielefeld University and has earned a Master?s degree in November
2010. Since then, she is an external PhD student at the Goettingen University and will finish her graduation with the topic ?Fermentation and formulation of an
entomopathogenic and endophytic B. bassiana strain? in 2014.
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