This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.
In this paper, we reported the construction of new high sensitive electrochemical genosensor based on multi-walled carbon
nanotubes-chitosan-bismuth complex (MWCNT-Chi-Bi) and lead sulfide nanoparticles for the detection of pathogenic
Aeromonas. Lead sulfide nanoparticles capped with 5-(NH2) oligonucleotides thought amide bond was used as signalizing probe
DNA (sz-DNA) and thiol-modified oligo nucleotides sequence was used as fixing probe DNA (fDNA). The two probes hybridize
with target Aeromonas DNA (tDNA) sequence (fDNA-tDNA-szDNA). The signal of hybridization is detected by differential pulse
voltammetry (DPV) after electro deposition of released lead nanoparticles (PbS) from sz-DNA on the surface of glass carbon
electrode decorated with MWCNT-Chi-Bi which improves the deposition and transducing electrical signal. A new ssDNA probe
and primers specific for the fragment Aerolysin gene (aer) was designed and the optimization of incubation time, hybridization
temperature, deposition potential, deposition time and the specificity of the probes were investigated. Our results showed the
highest sensibility to detect the target gene when compared with related biosensors and Polymerase Chain Reaction (PCR).
The detection limit for this biosensor was 10-14 M. We could detect lower than 102CFU mL-1 of Aeromonas in tap water. This
method is rapid and sensitive for the detection of pathogenic bacteria and would become a potential application in biomedical
diagnosis, food safety and environmental monitoring.
Antonio Maximiano Fernandes has completed his MSc at the age of 28 years from Jiangnan University. He is Medical Pathologist and Food Scientist Researcher,
developing the first real application of highly sensitive genosensor which has several applications. The main advantage of this research is the superior sensibility
application of nanomaterial in real samples detection. Recently he planned to combine electronics and real time samples detection and papers has been accepted
in high reputed journals such as Biosensor and Bioelectronic (UK) and Journal of Agriculture and Food Chemistry (USA).
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals