Diagnosis Of Aflatoxin Producing Fungi By A Sensitive PCR Assay In Food In Mashhad | 12211
Journal of Biotechnology & Biomaterials
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Aflatoxin contamination of consumable food commodities is a global health and economic risk. Aflatoxin B1 is a known
carcinogen and causes pleiotropic health problems in humans, necessitating its detection in commercial preparations of
food. Methodologies with exquisite sensitivities exist for the detection of aflatoxin. However, PCR based detection of Aspergillus
flavus, which produces aflatoxin B1, offers distinct advantages over traditional detection methods. We optimized methods for the
isolation of fungal genomic DNA from food commodities, established the sensitivity of a PCR assay (based on the detection of
AflR gene) and analyzed food items from local markets in Mashad area. The assay could identify A. flavus if 200 spores per gram
of food are present, which could indicate unfit nature of contaminated items for human consumption. However, based on our
analysis, all food items analyzed from Mashad area markets are free of A. flavus and therefore aflatoxin B1.
Fatemah Hashemi has completed her master at the age of 30 years from Ferdowsi University School of science. She is the researcher in Ferdowsi
University, has 2 papers ready for submission.
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