Differentiation Of Sheep And Goat Strains Of Mycobacterium Avium Subsp Paratuberculosis By Multiplex PCR On Large Sequence Polymorphisms | 4728
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
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Differentiation of sheep and goat strains of Mycobacterium avium subsp paratuberculosis by Multiplex PCR on Large sequence polymorphisms

3rd World Congress on Biotechnology

Radhika Syam

Posters: Agrotechnol

DOI: 10.4172/2155-952X.S1.020

Mycobacterium avium subsp paratuberculosis (MAP) is the causative agent of chronic wastin disease in cattle and sheep, but caused by different strains. Genomic reduction by loss of large sequence polymorphisms (LSPs) is the major contributor of genomic diversity in M avium subspecies and can also be used for differentiating cattle and sheep strains of MAP. A part of LSP A 4 is present only in sheep strains not in cattle strains. Faecal samples were collected from cattle, sheep and goat from Bareilly and Thrissur districts. DNA was extracted from those faecal samples by using the Qiagen QIAamp DNA kit and was subjected to IS 900 PCR. 20% cattle, 25% sheep and 44.44% goat were positive for MAP. All the DNA samples were subjected to LSP4 multiplex PCR for specific detection of cattle and sheep strains of MAP. All positive cattle samples had given amplification of 490 bp and all positive sheep samples had given amplification of 540 bp. One except all goat samples gave 540bp product indicating infection with sheep strain. One goat sample gave both 540bp and 490 bp products indicating mixed infection of sheep and goat strains as they are susceptible to both strains. None of the negative samples produced any amplification with LSP 4 multiplex PCR. The ability to detect and differentiate between strains of MAP is of obvious importance for both accurate diagnosis and to guide control programs. LSP?s are very specific for MAP diagnosis and strain differentiation of MAP.
Radhika Syam is the native of Kerala, India. She has completed her MVSc degree from Indian Veterinary research institute and is pursuing PhD from the same institute on Veterinary Bacteriology. She is the holder of JRF from ICAR for PG, ICMR and CSIR for PhD and SRF from ICAR for PhD. She is also honoured by Prime minister of India for her achievement in BVSc exams.