Dersleri yüzünden oldukça stresli bir ruh haline sikiş hikayeleri bürünüp özel matematik dersinden önce rahatlayabilmek için amatör pornolar kendisini yatak odasına kapatan genç adam telefonundan porno resimleri açtığı porno filmini keyifle seyir ederek yatağını mobil porno okşar ruh dinlendirici olduğunu iddia ettikleri özel sex resim bir masaj salonunda çalışan genç masör hem sağlık hem de huzur sikiş için gelip masaj yaptıracak olan kadını gördüğünde porn nutku tutulur tüm gün boyu seksi lezbiyenleri sikiş dikizleyerek onları en savunmasız anlarında fotoğraflayan azılı erkek lavaboya geçerek fotoğraflara bakıp koca yarağını keyifle okşamaya başlar
GET THE APP
Diversity Of Phosphate Solubilizing Bacteria From Proposed Uranium Mining Site | 4692
ISSN: 2155-952X
Journal of Biotechnology & Biomaterials
Open Access
Like us on:
Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
Radiological survey and exploration by Atomic Mineral Division (AMD), Hyderabad, has indicated the presence of rich
uranium deposits in the Bhima river belt of Karnataka (Gogi, Shahapur Taluk, Yadagiri district). The radionuclides/metal
enriched reserves are under-explored ecosystems in terms of microbial diversity. It is envisaged that, these ecosystems might
harbor microbes possessing special characteristics namely, metal-resistance, phosphate solubilization, and radiation resistance,
which may be of use in remediating metal contaminated sites. With this background information, the present study was aimed at
isolating and characterizing culturable and phosphate solubilizing bacteria from Gogi region (16? 43? N, 76? 44? E). Soil and tube
well water samples were collected aseptically from these sites. These samples were analyzed in laboratory for pH, temperature,
salinity, radionuclide activities namely K-40, Th-232 and Ra-226 and presence of phosphate solubilizing bacteria (PSB). For the
isolation of PSBs, the samples were inoculated on Pikovskaya?s agar and incubated for 10 days. Among 32 isolates obtained on this
media, 15 isolates showed clear zone around the colonies with the Solubilization Index > 1. These isolates were further assessed
for their solubilization efficiency in Pikovskaya?s broth containing 5 g/L tri-calcium phosphate for 3 days. The phosphate released
by the isolates ranged from 200 mg/L to 800 mg/L with proportional decrease in pH of the media which was significantly higher
compared to the uninoculated control. The decrease in pH can be attributed to the release of organic acids which play a role in
solubilization of insoluble phosphate. Taxonomic identification by 16S rRNA sequencing and phylogenetic analysis revealed that
the isolates belonged to eight genera namely Acinetobacter, Curtobacterium, Enterobacter, Leclercia, Pseudomonas, Pantoea,
Staphyloccus and Serratia. This study opens scope for in-depth investigation of these natural isolates possessing important
characteristics for environmental application.
Biography
Sowmya S has completed Masters degree in Biotechnology and is presently pursuing Ph.D. on the topic of bioremediation of uranium under the
guidance of Dr. Arun A.B. in Yenepoya University, Mangalore, Karnataka.
Relevant Topics
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals