Journal of Biotechnology & Biomaterials
Open Access

Abstract

Arsenic (As) is highly toxic, heavy metal present in environment due to natural and anthropogenic sources. Arsenic III is more toxic than Arsenic V. Ingestion of Arsenic III leads to increased risks of cancer, gastrointestinal symptoms and disturbances of cardiovascular, lungs, bladder and kidney and environmental pollution. Arsenic removal by biological means is an upcoming and eco-friendly technique where microorganisms have the ability to detoxify heavy metals from the environment. In this work, arsenic was detected from industrial effluents and was found to be significantly higher than WHO limits. Textile dye effluent showed highest arsenic concentration of 249 ppm. From these effluents, arsenic degrading bacteria were isolated and identified as Klebsiella pneumonae, Lysinibacillus fusiformis and Bacillus subtilis. The respective cultures were subjected to bioremediation and highest oxidation was found to be 80%. The effluents were also subjected to biosorption and phytoremediation. Highest biosorption rate was found to be 47.2% by chrome plating effluent isolate within 48 hours of incubation. Consortium of Klebsiella pneumoniae: Pseudomana putida showed 92.8% and Lysinibacillus fusiformis: Bacillus subtilis showed 94% degradation within 4 days. The dye isolate was subjected to synthesis of arsenic nanoparticle which could be exploited for drug delivery. All isolates showed the ability to produce siderophres. This work will help us in careful handling of issues like pollution, over exploitation of natural resources and sustainable development.

Biography

Sharad Ratan Khandelwal has completed his BSc, MSc and PhD degrees with merit at the Marathwada University, Aurangabad. He has 21 publications to his credit. He was nominated as a Member of Carrier Advancement Scheme (CAS) by Government of Maharashtra, Director, Higher and Technical Education Pune. He is a certified Auditor for ISO, Switzerland. He is working as a Chairman of MSc Microbiology Examination, University of Pune, Pune. He is honored to be a Member of Editorial Board for the International quarterly journal entitled Journal of Cancer and Therapy. He is also a recognized Guide for PhD/MPhil, University of Pune and YCMOU Nasik. Seven students have been awarded MPhil under his guidance and 6 PhD students are likely to register their names under University of Pune. He is also invited as Referee for PhD thesis evaluation from University of Mumbai and other universities. He has completed 5 minor, 1 major research projects from UGC New Delhi, 1 major project is underway and 1 project from BCUD University of Pune is also underway. He has so far guided 20 MSc dissertations successfully.

Email: Sharad_khandelwal13@yahoo.com