Journal of Biotechnology & Biomaterials
Open Access

Abstract

Centella asiatica is a perennial herb belonging to family Apiaceae and commonly known as ‘Indian pennywort’, ‘Mandookaparni’, ‘Jal brahmi’ or ‘Gotukola’. It is a creeping plant rooting at the nodes. This plant contains several important phytocompounds like triterpene acid, asiatic acid, madecassic acid etc. Asiaticoside is one of the important phytocompounds that helps in the treatment of jaundice, missiles, hepatitis, small pox and rheumatism. Due to the importance of asiaticoside, there is a need to micro-propagate this plant so that maximum yield of asiaticoside can be achieved. Various factors affect the yield of biomass and asiaticocides and in this study effects of different carbon sources and elicitors on the production of asiaticoside were observed. Explants were inoculated in Murashige and Skoog (MS) media supplemented with different carbon sources such as sucrose, glucose, maltose and fructose with varying concentration 2-5% and incubated at 25o C with 16 hours photoperiod. Highest shoot generation was observed with fructose. Different elicitors primarily malt extract, salicylic acid, jasmonic acid and methyl salicylate was also used to further enhance the production of asiaticoside.

Biography

Koyel Kundu is currently pursuing his MTech in Industrial Biotechnology in Delhi Technological University (Former Delhi College of Engineering). His area of interest is Plant Biotechnology and special focus is being done on medicinal plants.

Email: nabneetab@dtu.ac.in