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In Association with
Effect of high copy number of aprE gene on growth rate and sporulation frequency of the recombinant strain of Bacillus subtilis dnaC30 temperature sensitive mutant
8th Euro Biotechnology Congress
Malika Alili1 and Taha Zaghloul2
1Ecole Normale Superieure, Algeria 2University of Alexandria, Egypt
Posters-Accepted Abstracts: Biotechnol Biomater
Abstract
Plasmid p5.2 that carries the complete alkaline protease gene was used to transform Bacillus subtilis dnaC30 temperature sensitive mutant cells. This host is known to grow normally at 37 �?°C. However, when the temperature is changed to 48 �?°C (non permissive-temperature) the replication of the chromosome but not the plasmid is ceased. Consequently, this system allows the amplification of genes carried on plasmids. Growth rate and sporulation frequency of B. subtilis dnaC30 host cells and those of B. subtilis dnaC30 (p5.2) cells were studied. Data indicated that they were almost identical when both were grown at 37 �?°C. However, at non-permissive temperature the growth rate and sporulation frequency of B. subtilis dnaC30 host were reduced after 24 hours of incubation while those of B. subtilis dnaC30 (p5.2) were greatly affected. This could be due to the effect of the high copy number of the aprE gene at non-permissive temperature. Additionally, it can be stated that the characteristics of this mutant participated largely in this effectBiography
Email: amikalika@yahoo.fr
