Journal of Infectious Diseases & Therapy
Open Access

Abstract

Salmonella enterica is a Gram-negative bacterium responsible of salmonellosis, a gastrointestinal infection that causes 550 million cases each year, from which 220 million are children less than 5 years. In fact, S. enterica is one of four major etiological agent of diarrhea; worldwide. To cause infection, this bacterium contains a lot of virulence factors such as capsule, lipopolysaccharide, enterotoxins, and the capacity to forms biofilms. It has been reported that biofilm structure protects Salmonella from immune system cells and antibiotics action. Therefore, the development of new antimicrobials and anti-biofilms agents to combat salmonellosis are needed. In this work, we investigated the bactericide and biofilmicide effect of bLF and the peptides Lactoferricin17-30, D-Lactoferricin17-30, Lactoferrampin265-284, D-Lactoferrampin265-284 and LFchimera on Salmonella enterica. Materials and Methods: Strains of Salmonella enterica were incubated with bLF, LFcin17-30, D-LFcin17-30, LFampin265-284, D- LFampin265-284, LFchimera and gentamicin in different concentrations during 2 h. Then, the viability of cultures was assessed by determination of colony forming unit/ml. To form biofilms, strains of Salmonella were incubated for 12 (mature) or 8 (immature), and then; biofilms were treated with bLF, LFcin17-30, D-LFcin17-30, LFampin265-284, D-LFampin265-284, and LFchimera, during 4 and 6 h. The effect of treatment on biofilms was assessed by colony counts (CFU/ ml) and by Live/dead biofilms vitality kit. In addition; biofilms were visualized by florescence microscopy and fluorescence was measured. Results: bLF and synthetic peptides shown bactericide effect on salmonella, but bLF and LFchimera presented a greater effect. On the other hand bLF and LFchimera affected immature biofilms, but only bLF disrupted mature and established biofilms. Based in our results LF and LFchimera are alternative approaches that could prevent Salmonella colonization of the human host and perhaps against salmonellosis. Recent Publications 1. Leon-Sicairos, N., Angulo-Zamudio, U.A., Vidal, J.E., Lopez-Torres, C.A., Bolscher, J.G., Nazmi, K., Reyes-Cortes, R., Reyes-Lopez, M., De La Garza, M., and Canizalez-Roman, A. 2014 Bactericidal effect of bovine lactoferrin and synthetic peptide lactoferrin chimera in Streptococcus pneumoniae and the decrease in luxS gene expression by lactoferrin. Biometals 27: 969-980. 2. Baker, E.N., and Baker, H.M. 2009 A structural framework for understanding the multifunctional character of lactoferrin Biochimie 91: 3-10. 3. Bolscher, J., Nazmi, K., Van Marle, J., Van 'T Hof, W., and Veerman, E. 2012 Chimerization of lactoferricin and lactoferrampin peptides strongly potentiates the killing activity against Candida albicans Biochem Cell Biol 90: 378-388. 4. Leon-Sicairos, N., Angulo-Zamudio, U.A., Vidal, J.E., Lopez-Torres, C.A., Bolscher, J.G., Nazmi, K., Reyes-Cortes, R., Reyes-Lopez, M., De La Garza, M., and Canizalez-Roman, A. 2014. Bactericidal effect of bovine lactoferrin and synthetic peptide lactoferrin chimera in Streptococcus pneumoniae and the decrease in luxS gene expression by lactoferrin. Biometals 27, 969-980. 5. 5. Rodriguez, A., Pangloli, P., Richards, H.A., Mount, J.R., and Draughon, F.A. 2006. Prevalence of Salmonella in diverse environmental farm samples. J Food Prot 69, 2576-2580.

Biography

Nidia Leon-Sicairos has her expertise in evaluation of proteins and peptides with antimicrobial and antitumoral activities such as lactoferrin and derived peptides. Her research is focus in to describe the mechanism for which lactoferrin and its peptides exert its microbicidal and antitumoral effects in vitro and in vivo. The pathogens and tumoral cells target for these studies are those that cause infections in immunosuppressed patients, and in cells derived from leukemia, mama and lungs. The idea is to have new compounds that can help to patients in the fight against cancer and infections linked to this group of diseases.

E-mail: nidialeon@uas.edu.mx