Effects Of Conditions Of Culture On Mitochondrial Membrane Potential And Viability Of Genetically Modified Pichiapastoris | 17174
Journal of Biotechnology & Biomaterials
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genetically modified has been used as an efficient expression system for production of recombinant proteins.
For the production of scFv anti-LDL (-) has been used the methanol inducible promoter alcohol oxidase I. During the
induction phase, the conditions of culture such as temperature and concentration of methanol could affect to mitochondrial
function and viability of Pichiapastoris. The purpose of this study is to determine the effects of temperature and methanol
concentration on the mitochondrial function and viability of genetically modified
. We evaluated five different
conditions of culture: 14?C-1% (v/v) methanol (m), 14?C- 2%(v/v) m, 18?C-1.5% (v/v) m, 22?C-1% (v/v) m and 22?C-2%
(v/v) m. was analyzed the mitochondrial membrane potential using 5,5?,6,6?-tetrachloro-1,1?,3,3?-tetraethylbenzimidazolcarb
ocyanine iodide (JC-1) and the viability using propidium iodide by flow cytometer. The results to indicate that yeast cultured
during induction phase using less 2% (v/v) methanol improve the mitochondrial function and was observed diminution of
cell viability when yeast were exposed to 1.5% (v/v) or 2% (v/v) methanol. In conclusion, genetically modified
require low concentration of methanol for improve the energy production source and elevated levels of methanol could induce
cellular membrane damage.
Figueroa C A is studying his sandwich PhD in Universidad de La Frontera, Chile and Universidade de S?o Paulo, Brazil. She has published 5 papers and she is
member of Society of Pharmacology of Chile since 2012. She is developing her thesis in Biotechnological Pharmaceutics Laboratory of Universidade de S?o Paulo
and she is working with production of heterologous proteins in Pichiapastoris.
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