Effects Of Pentavalent Arsenic Toxicity On Germination, Seedling Growth And Peroxidase Activity In Black Eyed Bean | 4768
Journal of Biotechnology & Biomaterials
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Arsenic is one of the most toxic environmental pollutants because of its chronic and epidemic effects on human health through
widespread water and crop contamination. There are concerns that arsenic can be absorbed by plants and can bioaccumulate
in the food chain. Large numbers of studies indicated that low concentrations of arsenic can stimulate the growth of plants; but
excessive arsenic can be detrimental for plant growth and survival. The objective of the study was to investigate the effect of
pentavalent arsenic on the germination of Black eyed bean (Vigna unguiculata, variety: KH 11, PUSA), an important crop of
northern India. The germination of the seeds were studied in presence of sodium arsenate solutions (As V) (0.5 ppm., 1 ppm.,
1.5 ppm. and 2 ppm.). The sequestration of arsenic in different parts of the seedlings was estimated by using AAnalyst 200 atomic
absorption spectrometer (Perkin Elmer, MA, USA) fitted with a FIAS-100 flow injection system. The root and shoot lengths were
measured to study the changes in growth patterns. In order to assess the oxidative stress, Guiacol peroxidase (GPX) activity was
estimated in different parts of the seedlings (root, shoot and seed parts) and were expressed in enzyme activity/min./gm. fresh tissue.
The lengths of the roots and shoots of the seedlings increased significantly with the increase in concentrations of arsenic solutions
after seven days of incubation. Peroxidase activity was found to be highest in the root parts, followed by seed and shoot parts. One
possible reason for that is the direct exposure of the root and seed part with arsenic solutions, whereas shoot part emerges out of
the culture plate and thus was not under the direct exposure of arsenic. Interestingly, peroxidase activity gradually decreased in
the seed parts with increase in the concentrations of arsenic solutions, whereas in the root parts, peroxidase expressions decreased
upto 1.5 ppm. arsenic solution and then the activity further increased in presence of 2 ppm. arsenic solution. However, no specific
pattern of peroxidase expressions was found in the shoot parts. The study reveals that arsenic may have some interference with the
growth factors and enzyme systems in Black eyed bean, which deserves further attention in future researches.
Sayan Bhattacharya is a faculty in the department of Environmental Science, Asutosh College, Calcutta. For the last five years he has been
engaged in his doctoral research in Department of Environmental Science in University of Calcutta. He has published 17 International Conferences
proceedings and several National Conference proceedings, 6 book chapters and 5 international journal papers. He has received young researcher
awards from Govt. of India and IUPAC. He has more than 3 years of teaching experiences in 4 colleges and universities in West Bengal, India and
has experiences as a reviewer in international peer reviewed journals.
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