Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
Gold nano-particles have been known for their excellent biological compatibility, unique physico-chemical properties.
Nano-structured gold has been used in recognition of biological events, facilitating better electronic signal transduction,
leading to new generation of bio-electronic devices. We have fabricated gold nano aggregates (GNAs) based electrode via direct
electrodeposition method onto indium tin oxide coated glass plates for its application towards development of electrochemical
DNA biosensor for early detection of Typhoid. GNP-A/ITO electrode was characterized using X-Ray diffraction that reveals the
deposition of metallic gold nano particles in fcc structure. The GNAs/ITO electrode exhibited good electro-analytical activity and
shows quasi-reversible behaviour. GNAs/ITO electrode was modified with self assembled monolayer of 11-Mercaptoundecanoic
acid (MUA) to immobilize amine modified ssDNA probe for fabrication of ssDNA/MUA/GNAs/ITO bio-electrode. Response
studies of the ssDNA/MUA/GNAs/ITO bio-electrode towards target probes were done using differential pulse voltammetry
(DPV) by monitoring direct guanine oxidation and with ethylene blue (MB) as redox indicator. Direct monitoring of guanine
oxidation with complementary targets on ssDNA/MUA/GNP-A/ITO bio-electrode, limit of detection was found upto 150 ottomol
whereas Methylene blue redox indicator exhibited improved limit of detection upto 4 ottomol at 25�C in phosphate buffer with
in 60 s hybridization time. The bioelectrode shows 37 times enhanced sensitivity and was found efficiently able to distinguish
complementary, non-complementary and one base mismatch target. This biosensor electrode has implications towards detection
of other pathogens like M. tuberculosis, Campylobacter, H. Pylori, E. coli etc for clinical diagnostics, food quality control and
environmental monitoring.
Biography
Relevant Topics
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals