Electrochemical Impedance Spectroscopy (EIS) Biosensor For Detection Of Chinese Hamster Ovaryhost Cell Protein (CHO-HCP) Residues In Bio-therapeutics | 58939
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
Open Access

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Electrochemical impedance spectroscopy (EIS) biosensor for detection of Chinese hamster ovaryhost cell protein (CHO-HCP) residues in bio-therapeutics

12th Euro Biotechnology Congress

Khaled M Al-Qaoud

Yarmouk University, Jordan

Posters & Accepted Abstracts: J Biotechnol Biomater

DOI: 10.4172/2155-952X.C1.065

Production of recombinant therapeutic proteins using cell-based host expression system is regarded as the corner stone in the treatment of various life-threatening diseases including cancer and autoimmune diseases. About 70% of the recently approved therapeutic proteins are produced in Chinese hamster ovary (CHO) cell lines. However, the presence of host cell proteins (HCPs) as low as (1 to 100 ppm) will adversely affect the quality, safety and immunogenicity thus affecting the acceptance of the produced bio-therapeutic proteins. Searching for reliable, detectable and generic method for HCP detection is mandatory. In this regard, we assume that Electrochemical Impedance Spectroscopy (EIS) is the method of choice. Polyclonal rabbit antibody raised against CHO cell proteins extract were immobilized to a gold electrode that has been treated with 8-mercaptooctanoic acid and then activating the layer with EDC/NHS. Following the addition of CHO proteins and its further interaction with the rabbit antibodies, the charge transfer resistance is monitored. Notably the addition of successive concentrations of CHO protein extracts increased the charge transfer resistance of the electrochemical system. Results comparison with a commercial ELISA quantification system revealed consistent results with higher sensitivity using the electrochemical system. System validation using real and CHO-HCP-spiked therapeutics is in progress.

Khaled M Al-Qaoud was graduated in 1999 from the Bernhard Nocht Institute for Tropical Medicine in Hamburg and is working as a full Professor in the Department of Biological Sciences at Yarmouk University. He has published about 35 international publications and 3 patents in the field of camel antibody discovery and pharmaceutical usage. He succeeded in gaining good experience in linking the scientist in the academia with the industrial sector in the field of biotechnology, ended by the establishment of a biotech cluster of scientists from different disciplines.

Email: [email protected]