Epigenetic And OMICS Analyses For The Expression Control Of Secondary Metabolism Genes In Aspergillus Oryzae | 14113
Journal of Biotechnology & Biomaterials
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Filamentous fungi generally have various metabolic
pathways producing unique compounds utilizing variety
of starting materials. Of them, Aspergillus oryzae, which
are widely used in Japanese food industry has known
to produce kojic acid (KA) for many years in particular
conditions. Comparison of several genomes of Aspergilli
has shown two groups of genomes diff erent in size. Th e
bigger genomes were found to possess more metabolic
genes such as secretory hydrolases and secondary
metabolisms. Th e expression regulation of those genes
is of great interest because the former genes are useful
to degrade raw materials in fermentation and because
the latter genes are responsible to produce bio-active
compounds, which could be utilized in pharmaceutical
industry and could be essential to pathogenic ability.
However, large diversity and expression in limited
conditions of the genes have made their analyses
signifi cantly diffi cult. Recently, we have determined
genes responsible to KA biosynthesis. Th e gene cluster
consisted of three genes encoding oxidoreductase, a
transporter and a transcription regulator, which located
on the non-syntenic block. As a part of the studies to
improve KA productivity, we have analyzed epigenetic
regulation of the cluster by analyzing acetylation and
methylation of histone H3. Th e ChIP analysis using
anti-dimethyl H3 (lys4) and anti-acetyl H3 (lys9, 14)
indicated close relation between the modifi cations and
the expression levels of the genes for KA biosynthesis.
Based on the observations above, determination of
novel secondary metabolism genes by OMICS analyses
including transcriptome and extrolites analyses
in combination with bioinformatics is underway.
Th e results should be useful to further elucidate
transcriptional mechanism of secondary metabolism
Hiroko Hagiwara has completed his PhD from Saitama University and postdoctoral studies from Riken and AIST. She has contributed to genome sequencing and functional genomics of Saccharomyces cerevisiae and Aspergillus oryzae and to development of novel technologies useful in genomic studies including comprehensive screening method using bacteriophage and automated SNP detection method using paramagnetic beads.
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