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Evaluation of different reservoir souring control strategies in oil field simulating columns

6th World Congress on Biotechnology

Ganshyam Prajapat, Priyanka Kumari and Akhil Agrawal

Central University of Rajasthan, India

Posters-Accepted Abstracts: J Biotechnol Biomater

DOI: 10.4172/2155-952X.C1.044

Abstract
In oil reservoir, hydrogen sulfide is produced by sulfate reducing bacteria which has nasty odour. The production of hydrogen sulfide is called as reservoir souring. It is a common problem during secondary oil recovery when water is injected to produce the remaining oil from the oil reservoir. In the presence of electron donor in injection water, sulfate reducing bacteria (SRB) can reduce sulfate into sulfide. For the control of sulfide production, broad spectrum biocides can be used which control the microbial process. Use of nitrate as a biocide, directly affect the SRB population growth by numerous competitive and inhibitory mechanisms. The mechanism of souring control by nitate and biocide have been evaluated from oil field simulating sand packed columns. In our study, to simulate the oil reservoir environment, four up-flow sand packed-bed columns was used. To simulate waterflooding, synthetic modified Colville Synthetic Brine (CSB) media were pumped in the column and columns was inoculated with produced water sample of oil field. When nitrate was added in the CSB media, souring control was reported at injection port but not at outlet port due to total consumption of nitrate at the inlet port, so that inclusion of nitrate inhibits the SRB activity in inlet zone. In our study, Benzalkonium Chloride (BAC) is also used as biocide in reservoirsimulating columns in exclusively and combination way. BAC is involved in a gradual decrease in sulfide concentration. Continuous low nitrate and BAC concentration resultant was complete inhibition of SRB activity. Thus it was appeared that nitrate in combination with biocide is synergistic effect.
Biography

Ganshyam Prajapat has completed his Master’s degree in Microbiology from Central University of Rajasthan. He is currently pursuing his PhD from Central University of Rajasthan. His area of interest is in microbial souring, corrosion, control of biosouring, new biocide treatment and cyanobacterial UV-protecting molecules.

Email: 2014phdmb02@curaj.ac.in

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