Journal of Biotechnology & Biomaterials
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Mycobacterium avium subsp. paratuberculosis (Map) is the causative agent of johne's disease whose immunopathology
mainly depends on cell mediated immuneresponse. Genome sequencing revealed various PPE (Proline-Proline-Glutamic
acid) protein family of Map which are immunologically importance candidate genes In present study we have developed a
bicistrionic construct pIR PPE/IFN containing a 34.9
kDa PPE protein (PPE 34.9) of Map along with a cytokine gene encoding
murine gamma Interferon gene (IFNγ) and a monocistrionic construct pIR PPE using a mammalian vector system pIRES 6.1.
The construct were transfected in HeLa cell line and expression were studied by Western blot as well as Immunefluroscent assay
using recombinant sera. Further we have compared the immunereactivity of these two constructs in murine model by means of
DTH study, LTT, NO assay and ELISA. DTH response was higher in pIR PPE/IFN than pIR PPE group of mice, similar finding
also observed in case of LTT and NO production assay. ELISA titer of the pIR PPE/IFN was less than that with PPE only. These
preliminary finding can revealed a CMI response of this PPE protein of Map and IFNγ having synergistic effect on this PPE
Rajib Deb has completed his PG from Indian Veterinary Research Institute, Izatnagar, UP, India and was pursuing his PhD from same. Presently
he is working as Agriculture Research Scientist in Project directorate on cattle, Indian council of agriculture research, Meerut, UP, India. He has
published papers in reputed national and international journals.
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