Free Radical Scavenging, DNA Protection And Inhibition Of Lipid Peroxidation By Methanolic Extracts Of Elettaria Cardamomum And Ferula Assa-foetida | 38997
Journal of Biotechnology & Biomaterials
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Recent development in medicinal field reports a number of disease associated with free radicals. Antioxidants are the
substances which can scavenge free radicals and help to decrease the incidence of oxidative stress induced damage.
Therefore, present study was focused in search of natural antioxidants. For the study, methanolic extracts of Elettaria
cardamomum (EA) and Ferula assa-foetida (FA) were evaluated for antioxidant potential using TAC assay and reducing power
assay. Both the extracts showed antioxidant property and it was 25.60 mg/ml and 18.43 mg/ml ascorbic acid equivalents for
ET and FA respectively. Reducing power of FA (82.55%) was higher than EC (54.0%). The results of phytochemical screening
confirmed the presence of phenolics, alkaloids, flavonoids and saponin. TLC and HPLC were carried out to further analyze
these phytoconstituents. Total phenolic and flavonoid content was determined using gallic acid and quercetin as standard. Free
radical scavenging activity was evaluated using superoxide, hydroxyl radical scavenging and H2O2 decomposition assays. At a
dose 500 μg/ml FA and EC showed 82.55% and 54.0% scavenging of superoxide; 44.95% and 33.94% scavenging of hydroxyl;
22.34% and 17.65% decomposition of hydrogen peroxide respectively. Efforts were also made to study the effect on lipid
peroxidation and DNA damage-induced by hydroxyl radical using plasmid relaxation assay. EC and FA extracts significantly
inhibited lipid peroxidation in a dose dependent manner and also prevented DNA damage. All the results were compared with
standard antioxidants. Both extracts showed promising antioxidant and free radical scavenging activity, thus may be explored
as therapeutic agents for oxidative stress borne diseases in future.
Savita Chaurasia has completed her PhD in Medicinal Chemistry from Banaras Hindu University. She is a Professor of Biotechnology at IMS Engineering College, Ghaziabad. She has been serving as Editor-in-Chief of Vivechan International Journal of Research and has been serving as Associate Editor of Advances in Natural and Applied Sciences. She has been Member of RDC for Biotechnology/Bioinformatics of UP Technical University. She has been working towards the development of indigenous drugs of natural origin and application of these natural products as experimental medicines against various physiological and environmental challenges with an approach to their mechanism of action.