Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Frequency of PmrA, PmrB and mcr-1 genes in pseudomonas aeruginosa isolates from cystic fibrosis patients of mofid children hospital, Tehran, Iran

Joint Event on 3rd International Conference on Infection, Disease Control and Prevention & 2nd International Conference on Microbial Pathogenesis & Infectious Diseases

Mojdeh Hakemi Vala

Islamic Azad University, Iran

ScientificTracks Abstracts: J Infect Dis Ther

DOI: 10.4172/2332-0877-C2-041

Abstract
Introduction: The emergence of multidrug- resistant (MDR) in gram negative pathogens such as P. aeruginosa has become an important challenge to worldwide public health, recently (1). Colistin as a last line of treatment against MDR gram negative bacteria has been propounded (2). Recent studies have been demonstrated that colistin resistance is adaptive and regulated via PmrA and PmrB as a two component regulatory system (3). The plasmid borne colistin resistance gene mcr-1 was primarily recognized from China (4). Due to the raising of antibiotic resistance, in this study prevalence of mcr-1 gene and the mutation in pmrA and pmrB genes in P. aeruginosa isolates from sputum of Cystic Fibrosis (CF) patients was investigated. Methods: 41 isolates of P. aeruginosa from sputum of CF patients in Mofid Children Hospital during Apr-Sep 2017 were collected. According to CLSI guideline 2017, antibiotic susceptibility test (AST) was applied by using the disk diffusion method. Detection of pmrA, pmrB and mcr-1 genes was performed by PCR and further sequencing was administrated for finding the mutations. Results: Among 41 isolates of P. aeruginosa 22 (53.65%) were resistant to Amikacin, 21 (51.21%) to Ofloxacin and Cefepime, 20 (48.78%) to Imipenem, 19 (46.34%) to Ceftazidime and Ciprofloxacin, 18 (43.9%) to Aztreonam, 17 (41.46%) to Piperacillin, 16 (39.02%) to Gentamicin, 14 (34.14%) to Pipracillin-Tazobactam and 1 (2.43%) to Colistin. PCR results showed that all of the isolates had pmrA and pmrB genes and all of them were negative for mcr-1. One of the isolates show some mutations in pmrB gene. Conclusion: The results of this study showed that colistin is the best choice for treatment. Using the molecular tests is necessary and have a determinative role to prescription of antibiotics by physicians.
Biography

Mojdeh Hakemi Vala is working at Departmentof Microbiology, Medical school, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

E-mail: mojdeh_hakemi@yahoo.com

Top