Journal of Biotechnology & Biomaterials
Like us on:
Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
Jannik E. Jakobsen1, Morten M. Callesen, Tanja Ostergaard, Sira Lund, Martin Brorsb?l, Frederik Dagn?s-Hansen, Ying Liu, Rong Li, Mette Schmidt, Henrik
Callesen, S?ren H?yer, Christophe K. Mapendano, Torben F. Orntoft and Lars Dyrskj?t
Here we report the mouse Uroplakin-II promoter?s ability to drive transgene expression in the pig. Three different transposons
sizing 4.7 kb, 11.2 KB and 11.9 Kb were simultaneously transpositioned into primary fibroblasts, subsequently used
for somatic cell nuclear transfer to create various transgenic pigs harboring all three transposons. The pigs were made from
different transgenic fibroblast colonies and contained different copy numbers of each transposon. The two largest transposons
contain the Uroplakin-II promoter driving an array of genes including either the eGFP or RFP reporter gene linked to different
oncogenes and the rtTR-KRAB-2SM2 (KRAB-off) element. The pig with the highest copy number contained eighteen of these
large transposons whereas the pig with the lowest copy number contained three. Expression from the mouse Uroplakin-II
promoter was relatively week but enough to promote dysplasia in the bladder of a three month old pig. Some of the pigs are
now 4 months old and will be analyzed further using cystoscopy.
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals