Genetic Profiling Of Clinical Isolates Of Citrobacter Freundii Expressing BlaAmpC Gene In A Tertiary Referral Hospital Of Northern India | 40645
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
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Genetic profiling of clinical isolates of Citrobacter freundii expressing blaAmpC gene in a tertiary referral hospital of northern India

6th World Congress on Biotechnology

Shweta Mishra1, Supriya Upadhyay2 and Malay Ranjan Sen1

1Banaras Hindu University, India 2North Eastern Hill University, India

ScientificTracks Abstracts: J Biotechnol Biomater

DOI: 10.4172/2155-952X.C1.043

The common mechanism of resistance of Citrobacter freundii to β-lactam antibiotics is the presences of plasmid mediated blaAmpC. In this study we evaluate the emergence of ampC gene among clinical isolates of C. freundii isolated from urine of symptomatic UTI patients and also to assess mobile genetic elements. A total of 30 consecutive, clinical isolates of C. freundii were investigated for the presence of AmpC β-lactamase. Molecular characterization was studied by mPCR for gene of AmpC including the coproduction of other β-lactamase. Presence of integron was detected using integrase gene primers. Presence of integron to blaAmpC gene along with gene cassettes identified using specific primers. Genetic location on insertion sequence was also seen. A total of 16 isolates were phenotypically positive for AmpC. On performing their genotypic characterization, 5 isolates were found to be positive for blaCMY-2, 3 isolates having blaDHA-2 whereas one isolates coproducing blaCMY-2 and blaDHA-2. In which some AmpC positive isolates were coexisting blaNDM, blaIMP, blaVIM family of MBL and blaCTXM, blaSHV of ESBL. Among them 8 were harboring class 1 integron, linkage between integron and blaCMY-2 has been detected. Investigation of gene-cassettes revealed the presence of dihydrofolate reductase (dhfr) and aminoglycoside 6’-N-acetyltransferase (aac 6’) genes and presence of CMY-2 gene on ISEcp1 were also seen. The study showed that these genes have high ability to cross species barrier with ease and integrated other resistance genes. Eventually, AmpC gene highlights the potential risk in hospital environment. Thus the attainment of AmpC genes by C. freundii restricts therapeutic alternatives for combating infections.

Shweta Mishra has completed her PhD in 2014 from Department of Microbiology; Banaras Hindu University. She has achieved several fellowship programs and life member of premier organizations. She has her papers published in peer reviewed international journals like PLOSone, IJAA and IJMM and book chapters in international books and she is currently working as a Senior Research Fellow from ICMR funded project in the same department.

Email: [email protected]